AB6733

Rabbit Anti-Rat IgG H&L (Biotin)

Name: Rabbit Anti Rat (IgG) secondary antibody Biotin (ab6733) | Abcam
Brand: Abcam Limited
SKU: AB6733
Price: 265 USD
Availability: InStock

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(29 Publications)

Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Cited in 29 publications.

View Alternative Names

Ig gamma-1 chain C region

1 Images

Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (AB6733)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (AB6733)

ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

Dot, IHC-P, IHC-Fr, IM, ELISA, WB, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

NPJ biofilms and microbiomes 11:12 PubMed39794320

2025

Gut microbes modulate the effects of the flavonoid quercetin on atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuyuki Kasahara,Robert L Kerby,Ruben Aquino-Martinez,Abigail H Evered,Tzu-Wen L Cross,Jessica Everhart,Tyler K Ulland,Colin D Kay,Bradley W Bolling,Fredrik Bäckhed,Federico E Rey

Cell host & microbe 31:1038-1053.e10 PubMed37279756

2023

Gut bacterial metabolism contributes to host global purine homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuyuki Kasahara,Robert L Kerby,Qijun Zhang,Meenakshi Pradhan,Margarete Mehrabian,Aldons J Lusis,Göran Bergström,Fredrik Bäckhed,Federico E Rey

NPJ biofilms and microbiomes 9:31 PubMed37270570

2023

Dissecting the impact of dietary fiber type on atherosclerosis in mice colonized with different gut microbial communities.

Applications

Unspecified application

Species

Unspecified reactive species

Evan R Hutchison,Kazuyuki Kasahara,Qijun Zhang,Eugenio I Vivas,Tzu-Wen L Cross,Federico E Rey

Journal for immunotherapy of cancer 11: PubMed36914207

2023

Invasive margin tissue-resident macrophages of high CD163 expression impede responses to T cell-based immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Marit J van Elsas,Camilla Labrie,Anders Etzerodt,Pornpimol Charoentong,Jordi J C van Stigt Thans,Thorbald Van Hall,Sjoerd H van der Burg

Frontiers in immunology 14:1049713 PubMed36814928

2023

Pyrolyzed deketene curcumin controls regulatory T cell generation and gastric cancer metabolism cooperate with 2-deoxy-d-glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi MaruYama,Hirofumi Miyazaki,Yun-Ji Lim,Jian Gu,Masaki Ishikawa,Taichi Yoshida,WanJun Chen,Yuji Owada,Hiroyuki Shibata

Scientific reports 12:7407 PubMed35523907

2022

Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Delessard,Laura Stalin,Aurélie Rives-Feraille,Laura Moutard,Justine Saulnier,Ludovic Dumont,Nathalie Rives,Christine Rondanino

Histology and histopathology 37:587-595 PubMed35224715

2022

Cancer stem cell markers CD44v9+/CD133- are associated with low apoptosis in both sporadic and ulcerative colitis-associated colorectal cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Eriko Nakagomi,Tetuo Mikami,Kimihiko Funahashi,Shinichi Okazumi,Kazutoshi Shibuya,Nobuyuki Hiruta,Yoshinori Igarashi

Cellular and molecular gastroenterology and hepatology 13:405-423 PubMed34624527

2021

Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yeshwant Kurhe,Mara Caputo,Emmelie Cansby,Ying Xia,Sima Kumari,Sumit Kumar Anand,Brian W Howell,Hanns-Ulrich Marschall,Margit Mahlapuu

iScience 24:103241 PubMed34755089

2021

The ubiquitin ligase HOIL-1L regulates immune responses by interacting with linear ubiquitin chains.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Gomez-Diaz,Gustav Jonsson,Katrin Schodl,Luiza Deszcz,Annika Bestehorn,Kevin Eislmayr,Jorge Almagro,Anoop Kavirayani,Mayu Seida,Lilian M Fennell,Astrid Hagelkruys,Pavel Kovarik,Josef M Penninger,Fumiyo Ikeda

Infection and immunity 89:e0034621 PubMed34516235

2021

Mucosal Immunization with DTaP Confers Protection against Infection and Cough in Sprague-Dawley Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse M Hall,Graham J Bitzer,Megan A DeJong,Jason Kang,Ting Y Wong,M Allison Wolf,Justin R Bevere,Mariette Barbier,F Heath Damron

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Rat IgG H&L (Biotin)

Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (AB6733)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (29)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise