AB102250

Rabbit Anti-Rat IgG H&L (Biotin) preadsorbed

Name: Rabbit Anti Rat (IgG) secondary antibody preadsorbed Biotin (ab102250) | Abcam
Brand: Abcam Limited
SKU: AB102250
Price: 295 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Suitable for WB, ELISA, IHC-P, ICC. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

ELISA, IHC-P, WB, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Chemiluminescent)." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Primary)." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, horse, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Rat IgG H&L (Biotin) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise