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Suitable for ICC, ELISA, IHC-P, WB. Ideal for western blot. Cited in 3 publications.

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Images

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB102172), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
ICC, ELISA, IHC-P, WB
Clonality
Polyclonal

Reactivity data

Application
ICC
Reactivity
Reacts
Dilution info
1/200.00000 - 1/5000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/100000.00000
Notes

(as primary)

Application
IHC-P
Reactivity
Reacts
Dilution info
1/200.00000 - 1/5000.00000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000.00000 - 1/20000.00000
Notes

(Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).

Target data

Alternative names

Ig gamma-1 chain C region

Recommended products

Suitable for ICC, ELISA, IHC-P, WB. Ideal for western blot. Cited in 3 publications.

Key facts

Description
Rabbit Anti-Rat IgG H&L (HRP)
Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
ICC, ELISA, IHC-P, WB
Clonality
Polyclonal
Pre-adsorbed
No
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Part of the AbExcel range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172), expandable thumbnail

    Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172)

    Lanes 1 - 6: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1 µg/mL

    Lane 5: Anti-RUNX2 antibody (ab102712) at 1/20000 dilution

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/2000 dilution

    Lanes 3 - 4: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/10000 dilution

    Lane 6: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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