AB102172

Rabbit Anti-Rat IgG H&L (HRP)

Name: Rabbit Anti Rat (IgG) secondary antibody HRP (ab102172) | Abcam
Brand: Abcam Limited
SKU: AB102172
Price: 265 USD
Availability: InStock

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(3 Publications)

Suitable for ICC, ELISA, IHC-P, WB. Ideal for western blot. Cited in 3 publications.

View Alternative Names

Ig gamma-1 chain C region

1 Images

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Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB102172)

Lanes 1 - 6:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1 µg/mL

Lane 5:

Anti-RUNX2 antibody (<a href='/en-us/products/unavailable/runx2-antibody-ab102712'>ab102712</a>) at 1/20000 dilution

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/2000 dilution

Lanes 3 - 4:

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/10000 dilution

Lane 6:

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

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Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

WB, IHC-P, ELISA, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(as primary)" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"(Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000)." } } }

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Product details

Part of the AbExcel range.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 93: PubMed31341054

2019

Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurore Bochnakian,Anjie Zhen,Dimitrios G Zisoulis,Adam Idica,Vineet N KewalRamani,Nicholas Neel,Iben Daugaard,Matthias Hamdorf,Scott Kitchen,KyeongEun Lee,Irene Munk Pedersen

International journal of molecular sciences 20: PubMed31010097

2019

miR-128 Restriction of LINE-1 (L1) Retrotransposition Is Dependent on Targeting hnRNPA1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Lianna Fung,Herlinda Guzman,Evgueni Sevrioukov,Adam Idica,Eddie Park,Aurore Bochnakian,Iben Daugaard,Douglas Jury,Ali Mortazavi,Dimitrios G Zisoulis,Irene M Pedersen

The Journal of biological chemistry 292:20494-20508 PubMed28974576

2017

MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Idica,Evgueni A Sevrioukov,Dimitrios G Zisoulis,Matthias Hamdorf,Iben Daugaard,Pavan Kadandale,Irene M Pedersen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Rat IgG H&L (HRP)

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB102172)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

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Product promise