Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 120 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/170000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Ig gamma-1 chain C region
Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 120 publications
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
This antibody reacts with the heavy and light chains of Rat IgG
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Lanes 1 and 3: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/5000 dilution
Lanes 2 and 4: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/10000 dilution
Lanes 1 - 2: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Lanes 3 - 4: Western blot - BALB/3T3 whole cell lysate (BALB/3T3 whole cell lysate ab7901) at 20 µg
All lanes: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 17 kDa, 34 kDa, 52 kDa, 80 kDa
Exposure time: 10s
IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ELISA results of ab6734 against purified Rat IgG protein. Each well was coated in duplicate with 1.0 µg of Rat IgG [Green Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/41,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
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