Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 120 publications
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- Cellular and molecular life sciences : CMLS 81:2292024Dynamic changes in RNA mA and 5 hmC influence gene expression programs during macrophage differentiation and polarisation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Pinello,Renhua Song,Quintin Lee,Emilie Calonne,Kun-Long Duan,Emilie Wong,Jessica Tieng,Majid Mehravar,Bowen Rong,Fei Lan,Ben Roediger,Cheng-Jie Ma,Bi-Feng Yuan,John E J Rasko,Mark Larance,Dan Ye,François Fuks,Justin J-L WongPubMed 38780787
- Heliyon 9:e215492023Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengTao Hao,Chao Yin,XiaoLong Wang,ZhiQi Huo,GuoRong Zhang,Dong Jiang,Min AnPubMed 38027809
- Cellular & molecular biology letters 28:872023Cadmium promotes nonalcoholic fatty liver disease by inhibiting intercellular mitochondrial transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Sun,Yan Chen,Tao Wang,Waseem Ali,Yonggang Ma,Yan Yuan,Jianhong Gu,Jianchun Bian,Zongping Liu,Hui ZouPubMed 37884867
- Scientific reports 13:164242023Melatonin loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles reduce inflammation, inhibit apoptosis and protect rat's liver from the hazardous effects of CCL4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyaa Farid,Valina Michael,Gehan SafwatPubMed 37777583
- Asian biomedicine : research, reviews and news 17:22-292023Factors affecting the shear wave elastic quantitative measurement of penile tissue in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan-Ting Rao,Jing-Dong Tang,Jin-Fang XingPubMed 37551198
- Methods in molecular biology (Clifton, N.J.) 2690:23-362023Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRucha Karnik,Michael R BlattPubMed 37450134
- Scientific reports 13:106172023Therapeutic effect of melatonin-loaded chitosan/lecithin nanoparticles on hyperglycemia and pancreatic beta cells regeneration in streptozotocin-induced diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHabiba Alaa,Mariam Abdelaziz,Maryam Mustafa,Mustafa Mansour,Salma Magdy,Salma Mohsen,Yomna El-Karamany,Alyaa FaridPubMed 37391460
- Nature communications 14:36592023The vacuolar iron transporter mediates iron detoxification in Toxoplasma gondii.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDana Aghabi,Megan Sloan,Grace Gill,Elena Hartmann,Olga Antipova,Zhicheng Dou,Alfredo J Guerra,Vern B Carruthers,Clare R HardingPubMed 37339985
- Molecular medicine reports 28:2023Construction of ceRNA regulatory networks for osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongtao Chen,Hailong Wang,Xu Liu,Long Li,Yiliyaer Abudusimu,Yilihamu TuohetiPubMed 37326104
- Open medicine (Warsaw, Poland) 18:202306342023F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Sun,Lili He,Peiran Guo,Fenghua Li,Bo Wang,Yifan Zhang,Kai An,Ming PengPubMed 37082613
Key facts
- Host species
- Rabbit
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- IHC-Fr, IHC-P, ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/170000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 120 publications
Key facts
- Description
- Rabbit Anti-Rat IgG H&L (HRP)
- Host species
- Rabbit
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- IHC-Fr, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the heavy and light chains of Rat IgG
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4 product images
Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
Lanes 1 and 3: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/5000 dilution
Lanes 2 and 4: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/10000 dilution
Lanes 1 - 2: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Lanes 3 - 4: Western blot - BALB/3T3 whole cell lysate (BALB/3T3 whole cell lysate ab7901) at 20 µg
SecondaryAll lanes: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 17 kDa, 34 kDa, 52 kDa, 80 kDa
Exposure time: 10s
Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ELISA - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
ELISA results of ab6734 against purified Rat IgG protein. Each well was coated in duplicate with 1.0 µg of Rat IgG [Green Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/41,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
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