Rabbit Anti-Rat IgG H&L (HRP)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ELISA

Supplier Data

ELISA - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

ELISA results of ab6734 against purified Rat IgG protein. Each well was coated in duplicate with 1.0 µg of Rat IgG [Green Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/41,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

• WB

Unknown

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

Lanes 1 and 3:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1/5000 dilution

Lanes 2 and 4:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1/10000 dilution

Lanes 1 - 2:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

Lanes 3 - 4:

Western blot - BALB/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/balb-3t3-whole-cell-lysate-ab7901'>ab7901</a>) at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 17 kDa,34 kDa,52 kDa,80 kDa

false

Exposure time: 10s

• IHC-Fr

AbReview30384****

Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.

Secondary

All lanes:

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

false