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Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.

- Cited in over 120 publications


Images

Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB6734), expandable thumbnail
  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (AB6734), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (AB6734), expandable thumbnail
  • ELISA - Rabbit Anti-Rat IgG H&L (HRP) (AB6734), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
IHC-Fr, IHC-P, ELISA, WB
Clonality
Polyclonal

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Reactivity data

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/170000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Alternative names

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Rabbit Anti-Rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ICC/IF, ELISA and more.

- Cited in over 120 publications

Key facts

Description
Rabbit Anti-Rat IgG H&L (HRP)
Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
IHC-Fr, IHC-P, ELISA, WB
Clonality
Polyclonal
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This antibody reacts with the heavy and light chains of Rat IgG

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4 product images

  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734), expandable thumbnail

    Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

    Lanes 1 and 3: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/5000 dilution

    Lanes 2 and 4: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1/10000 dilution

    Lanes 1 - 2: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

    Lanes 3 - 4: Western blot - BALB/3T3 whole cell lysate (BALB/3T3 whole cell lysate ab7901) at 20 µg

    Secondary

    All lanes: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution

    Predicted band size: 50 kDa

    Observed band size: 17 kDa, 34 kDa, 52 kDa, 80 kDa

    Exposure time: 10s

  • Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734), expandable thumbnail

    Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

    ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.

    Secondary

    All lanes: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

    IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ELISA - Rabbit Anti-Rat IgG H&L (HRP) (ab6734), expandable thumbnail

    ELISA - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)

    ELISA results of ab6734 against purified Rat IgG protein. Each well was coated in duplicate with 1.0 µg of Rat IgG [Green Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/41,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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