Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
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- Toxicologic pathology 49:755-7722020Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Chlipala,Mark Butters,Miles Brous,Jessica S Fortin,Roni Archuletta,Karen Copeland,Brad BolonPubMed 33251977
- International journal of molecular sciences 21:2020A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep Dhall et. al.PubMed 32545915
- Blood 135:1969-19822020Platelet protein S limits venous but not arterial thrombosis propensity by controlling coagulation in the thrombus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Calzavarini,Raja Prince-Eladnani,François Saller,Luca Bologna,Laurent Burnier,Anne C Brisset,Claudia Quarroz,Maria Desiré Reina Caro,Vladimir Ermolayev,Yasuhiro Matsumura,José A Fernández,Tilman M Hackeng,John H Griffin,Anne Angelillo-ScherrerPubMed 32276277
- Molecular cancer therapeutics 18:1544-15542019Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Ziffels et. al.PubMed 31213507
- Scientific reports 9:23242019Transcriptional regulation of Hepatic Stellate Cell activation in NASH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnn-Britt Marcher et. al.PubMed 30787418
- Journal of neuroinflammation 14:592017The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Jendresen et. al.PubMed 28320424
Key facts
- Host species
- Rabbit
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Horse, Cow, Human
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/30000.00000 | Notes Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution. |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/30000.00000 | Notes Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution. |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
Key facts
- Description
- Rabbit Anti-Rat IgG H&L preadsorbed
- Host species
- Rabbit
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Horse, Cow, Human
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Ubiquitin (phospho S65) with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 at 1/5000 (0.179 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 for 30 mins at room temperature and followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded Mouse Alzheimer's Disease (FAD4T model) brain tissue labeling Ubiquitin (phospho S65) with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 at 1/5000 (0.179 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse Alzheimer's Disease (FAD4T model) brain without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 for 30 mins at room temperature and followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's Disease brain tissue labeling Ubiquitin (phospho S65) with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 at 1/5000 (0.179 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human Alzheimer's Disease brain without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Ubiquitin (phospho S65) antibody [41H1/41K3] - Rat IgG2a (Chimeric) ab318991 for 30 mins at room temperature and followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling CD16 with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 at 1/1000 (0.93 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of human ovarian cancer.
The section was incubated with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 for 10 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD16 with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 at 1/1000 (0.93 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen.
The section was incubated with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 for 10 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L preadsorbed (ab102248)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD16 with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 at 1/1000 (0.93 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on Kuffer cells of human liver.
The section was incubated with Anti-CD16 antibody [SP175] - Rat IgG2a (Chimeric) ab308607 for 10 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins.
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