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AB7099

Rabbit Anti-Rat IgG H&L preadsorbed

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(5 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma-1 chain C region

1 Images
Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (AB7099)
  • WB

Supplier Data

Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (AB7099)

Western blot analysis of ab7099. Secondary antibody : Anti-Rabbit IgG (Goat) Peroxidase Conjugated Antibody at 1/40000 dilution in for 30 min at RT.

All lanes:

Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (ab7099) at 1/1000 dilution

Lane 1:

Rat IgG at 50 ng

Lane 2:

Rat IgG F(c) Fragment at 50 ng

Lane 3:

Rat IgG Fab Fragment at 50 ng

Lane 4:

Rat IgM Whole Molecule at 50 ng

Lane 5:

Rat Serum at 50 ng

Secondary

All lanes:

Anti-Rabbit IgG at 1/40000 dilution

false

Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Applications

IHC-Fr, ID, ELISA, ICC/IF, WB, Conjugation, IP, IHC-P

applications

Clonality

Polyclonal

Immunogen

Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region.

P20759

Specificity

No reaction was observed against Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/300000 - 1/1400000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/15000 - 1/75000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:379 PubMed34876558

2021

Oncogenic microRNA-181d binding to OGT contributes to resistance of ovarian cancer cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Ling Chen,Kean Zhu,Donglian Wang

Frontiers in physiology 11:1092 PubMed33192538

2020

OGT-Mediated KEAP1 Glycosylation Accelerates NRF2 Degradation Leading to High Phosphate-Induced Vascular Calcification in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Hua Xu,Yinke Du,Zitong Sheng,Yue Li,Xiaobo Qiu,Binyao Tian,Li Yao

Journal of biological engineering 13:60 PubMed31303891

2019

Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of .

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Wang Zhang,Xia Wu,Dan Liu,Wei Zhou,Wei Tan,Yu-Xuan Fang,Yu Zhang,Yan-Qing Liu,Guo-Qing Li

PloS one 11:e0165883 PubMed27832099

2016

Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells - Relevance to Capsular Opacification.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Recek,Sofija Andjelić,Nataša Hojnik,Gregor Filipič,Saša Lazović,Alenka Vesel,Gregor Primc,Miran Mozetič,Marko Hawlina,Goran Petrovski,Uroš Cvelbar

PloS one 10:e0131702 PubMed26114879

2015

AIM2 Drives Joint Inflammation in a Self-DNA Triggered Model of Chronic Polyarthritis.

Applications

Unspecified application

Species

Mouse

Christopher Jakobs,Sven Perner,Veit Hornung
View all publications
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Product promise

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