Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Conjugation | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application ID | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000 - 1/10000 | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/300000 - 1/1400000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/15000 - 1/75000 | Notes - |
Ig gamma-1 chain C region
Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
No reaction was observed against Human Serum Proteins.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Terms & Conditions.
Western blot analysis of ab7099. Secondary antibody: Anti-Rabbit IgG (Goat) Peroxidase Conjugated Antibody at 1/40000 dilution in for 30 min at RT.
All lanes: Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (ab7099) at 1/1000 dilution
Lane 1: Rat IgG at 50 ng
Lane 2: Rat IgG F(c) Fragment at 50 ng
Lane 3: Rat IgG Fab Fragment at 50 ng
Lane 4: Rat IgM Whole Molecule at 50 ng
Lane 5: Rat Serum at 50 ng
All lanes: Anti-Rabbit IgG at 1/40000 dilution
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