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Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

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Images

Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (AB7099), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Applications
Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF
Clonality
Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759

Reactivity data

Application
Conjugation
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
IP
Reactivity
Expected
Dilution info
-
Notes

-

Application
ID
Reactivity
Expected
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000 - 1/10000
Notes

-

Application
ELISA
Reactivity
Expected
Dilution info
1/300000 - 1/1400000
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/15000 - 1/75000
Notes

-

Alternative names

Recommended products

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

Key facts

Description
Rabbit Anti-Rat IgG H&L preadsorbed
Host species
Rabbit
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Applications
Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
Yes
Immunogen
  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759
Specificity

No reaction was observed against Human Serum Proteins.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (ab7099), expandable thumbnail

    Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (ab7099)

    Western blot analysis of ab7099. Secondary antibody: Anti-Rabbit IgG (Goat) Peroxidase Conjugated Antibody at 1/40000 dilution in for 30 min at RT.

    All lanes: Western blot - Rabbit Anti-Rat IgG H&L preadsorbed (ab7099) at 1/1000 dilution

    Lane 1: Rat IgG at 50 ng

    Lane 2: Rat IgG F(c) Fragment at 50 ng

    Lane 3: Rat IgG Fab Fragment at 50 ng

    Lane 4: Rat IgM Whole Molecule at 50 ng

    Lane 5: Rat Serum at 50 ng

    Secondary

    All lanes: Anti-Rabbit IgG at 1/40000 dilution

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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