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AB150181

Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488)

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(9 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 9 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150181 Alexa Fluor® 488 rabbit anti-sheep IgG (H+L) (green) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)

Key facts

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, ICC/IF, Flow Cyt, IHC-Fr, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 96:102139 PubMed40189098

2025

Deubiquitinating enzyme USP2 regulates brown adipose tissue thermogenesis via controlling EBF2 stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejie Xu,Ying Chen,Ningning Bai,Yingying Su,Yafen Ye,Rong Zhang,Ying Yang,Caizhi Liu,Cheng Hu,Jiemin Pan

Experimental animals 73:293-301 PubMed38369347

2024

Progranulin deficiency attenuates tubulointerstitial injury in a mouse unilateral ureteral obstruction model.

Applications

Unspecified application

Species

Unspecified reactive species

Eri Adachi,Maki Murakoshi,Terumi Shibata,Kenta Shimozawa,Hiroko Sakuma,Chiaki Kishida,Tomohito Gohda,Yusuke Suzuki

Signal transduction and targeted therapy 8:225 PubMed37280192

2023

Adult-born neurons in critical period maintain hippocampal seizures via local aberrant excitatory circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Chen,Yingwei Xu,Heming Cheng,Zhongxia Li,Nanxi Lai,Menghan Li,Yeping Ruan,Yang Zheng,Fan Fei,Cenglin Xu,Jiao Ma,Shuang Wang,Yan Gu,Feng Han,Zhong Chen,Yi Wang

Frontiers in neuroscience 17:1145079 PubMed37034166

2023

Activation of POMC neurons to adiponectin participating in EA-mediated improvement of high-fat diet IR mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wanling Xu,Junfeng Li,Chang Ji,Danwei Fang,Lulu Yao,Nenggui Xu,Wei Yi

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21227 PubMed33247500

2020

Unexpected obesity, rather than tumorigenesis, in a conditional mouse model of mitochondrial complex II deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Fatimah Al Khazal,Seungwoo Kang,Molly Nelson Holte,Doo-Sup Choi,Ravinder Singh,Patricia Ortega-Sáenz,José López-Barneo,L James Maher

Journal of neurotrauma 38:353-364 PubMed32977735

2020

Regulates the NF-κB Signaling Pathway of Natural Stem Cells to Modulate Macrophage Migration during Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Li,Zi-Jie Rong,Yong Cao,Li-Yuan Jiang,Dong Zhong,Cheng-Jun Li,Xiao-Long Sheng,Jian-Zhong Hu,Hong-Bin Lu

Nature neuroscience 20:1734-1743 PubMed29184209

2017

A craniofacial-specific monosynaptic circuit enables heightened affective pain.

Applications

Unspecified application

Species

Unspecified reactive species

Erica Rodriguez,Katsuyasu Sakurai,Jennie Xu,Yong Chen,Koji Toda,Shengli Zhao,Bao-Xia Han,David Ryu,Henry Yin,Wolfgang Liedtke,Fan Wang

Molecular medicine reports 16:9473-9479 PubMed29152658

2017

Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wu,Jing Tang,Yi Li,Li Li,Yujia Wang,Ji Bao,Hong Bu

Human molecular genetics 26:4095-4104 PubMed28985353

2017

Loss of ADAMTS3 activity causes Hennekam lymphangiectasia-lymphedema syndrome 3.

Applications

Unspecified application

Species

Unspecified reactive species

Pascal Brouillard,Laura Dupont,Raphael Helaers,Richard Coulie,George E Tiller,Joseph Peeden,Alain Colige,Miikka Vikkula
View all publications

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