Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488)
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(9 Publications)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150181 Alexa Fluor® 488 rabbit anti-sheep IgG (H+L) (green) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- Alexa Fluor®
Unknown
Alexa Fluor® - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 488) (AB150181)
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Publications (9)
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Molecular metabolism 96:102139 PubMed40189098
2025
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Experimental animals 73:293-301 PubMed38369347
2024
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Signal transduction and targeted therapy 8:225 PubMed37280192
2023
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Frontiers in neuroscience 17:1145079 PubMed37034166
2023
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21227 PubMed33247500
2020
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Journal of neurotrauma 38:353-364 PubMed32977735
2020
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Nature neuroscience 20:1734-1743 PubMed29184209
2017
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Molecular medicine reports 16:9473-9479 PubMed29152658
2017
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Human molecular genetics 26:4095-4104 PubMed28985353
2017
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