Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 594)
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(1 Publication)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150184)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150184 Alexa Fluor® 594 rabbit anti-sheep IgG (H+L) (orange) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
- Alexa Fluor®
Unknown
Alexa Fluor® - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150184)
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Publications (1)
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eNeuro 3: PubMed27957527
2016
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