Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 647)
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Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging.
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Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150183)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150183 Alexa Fluor® 647 rabbit anti-sheep IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- Alexa Fluor®
Unknown
Alexa Fluor® - Rabbit Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150183)
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