AB6746

Rabbit Anti-Sheep IgG H&L (Biotin)

Name: Rabbit Anti Sheep (IgG) secondary antibody Biotin (ab6746) | Abcam
Brand: Abcam Limited
SKU: AB6746
Price: 265 USD
Availability: InStock

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(7 Publications)

Rabbit Anti-Sheep IgG H&L (Biotin)(ab6746) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.

Key facts

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

ELISA, IHC-P, ICC/IF, IHC-Fr, Dot, IM, WB

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/3000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/140000", "notes":"" } } }

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Product details

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Proceedings of the National Academy of Sciences of 117:6792-6800 PubMed32152097

2020

Intestinal bile acids directly modulate the structure and function of TcdB toxin.

Applications

Unspecified application

Species

Unspecified reactive species

John Tam,Simoun Icho,Evelyn Utama,Kathleen E Orrell,Rodolfo F Gómez-Biagi,Casey M Theriot,Heather K Kroh,Stacey A Rutherford,D Borden Lacy,Roman A Melnyk

Frontiers in microbiology 10:2790 PubMed31849920

2019

Omeprazole Increases the Efficacy of Acyclovir Against Herpes Simplex Virus Type 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Michaelis,Malte C Kleinschmidt,Denisa Bojkova,Holger F Rabenau,Mark N Wass,Jindrich Cinatl

Technology in cancer research & treatment 18:1533033819864311 PubMed31405336

2019

Inhibition of CD133 Overcomes Cisplatin Resistance Through Inhibiting PI3K/AKT/mTOR Signaling Pathway and Autophagy in CD133-Positive Gastric Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Lu,Gang Zhao,Yulong Yang,Zhaoyan Jiang,Jingli Cai,Hai Hu

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:14739-51 PubMed25355226

2014

BDNF-dependent plasticity induced by peripheral inflammation in the primary sensory and the cingulate cortex triggers cold allodynia and reveals a major role for endogenous BDNF as a tuner of the affective aspect of pain.

Applications

IHC

Species

Rat

Karine Thibault,Wee Khang Lin,Armelle Rancillac,Marie Fan,Thibaut Snollaerts,Vallier Sordoillet,Michel Hamon,George M Smith,Zsolt Lenkei,Sophie Pezet

Proceedings of the National Academy of Sciences of 110:12042-7 PubMed23818620

2013

Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Periz,Cristina Celma,Bo Jing,Justin N M Pinkney,Polly Roy,Achillefs N Kapanidis

The Journal of biological chemistry 280:5703-15 PubMed15590652

2004

p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Evo Lindersson,Ditte Lundvig,Christine Petersen,Peder Madsen,Jens R Nyengaard,Peter Højrup,Torben Moos,Daniel Otzen,Wei-Ping Gai,Peter C Blumbergs,Poul Henning Jensen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Sheep IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise