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AB6743

Rabbit Anti-Sheep IgG H&L (FITC)

5

(1 Review)

|

(11 Publications)

Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, Dot, ELISA. Cited in 11 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images
Dot Blot - Rabbit Anti-Sheep IgG H&L (FITC) (AB6743)
  • Dot

Supplier Data

Dot Blot - Rabbit Anti-Sheep IgG H&L (FITC) (AB6743)

Dot blot analysis of ab6743. Imaged with BioRad ChemiDoc, Fluorescein filter.

All lanes:

Dot Blot - Rabbit Anti-Sheep IgG H&L (FITC) (ab6743)

Lane 1:

Sheep IgG at 100 ng

Lane 2:

Sheep IgG at 33.3 ng

Lane 3:

Sheep IgG at 11.1 ng

Lane 4:

Sheep IgG at 3.7 ng

Lane 5:

Sheep IgG at 1.23 ng

Secondary

All lanes:

Rabbit Anti-Sheep IgG Antibody FITC at 1 µg/mL

false

Key facts

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Dot, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF, IM

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" } } }
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Product details

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Sheep IgG
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Experimental cell research 370:506-518 PubMed30031130

2018

FPR1 gene silencing suppresses cardiomyocyte apoptosis and ventricular remodeling in rats with ischemia/reperfusion injury through the inhibition of MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Ling Zhou,Fei Teng,Yong-Shan Zhang,Qiang Sun,Yan-Xia Cao,Guo-Wei Meng

Biomedical reports 6:627-632 PubMed28584633

2017

Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Tang,Qiong Wu,Yi Li,Xiujuan Wu,Yujia Wang,Lihua Zhu,Yujun Shi,Hong Bu,Ji Bao,Mingjun Xie

Immunity 46:379-392 PubMed28329704

2017

The IFN-λ-IFN-λR1-IL-10Rβ Complex Reveals Structural Features Underlying Type III IFN Functional Plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Juan L Mendoza,William M Schneider,Hans-Heinrich Hoffmann,Koen Vercauteren,Kevin M Jude,Anming Xiong,Ignacio Moraga,Tim M Horton,Jeffrey S Glenn,Ype P de Jong,Charles M Rice,K Christopher Garcia

PloS one 9:e90737 PubMed24595072

2014

γ-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Corina Wilding,Katharina Bell,Sabine Beck,Sebastian Funke,Norbert Pfeiffer,Franz H Grus

Analytical chemistry 85:8773-9 PubMed23952579

2013

Cardiac cell culture model as a left ventricle mimic for cardiac tissue generation.

Applications

Unspecified application

Species

Unspecified reactive species

Mai-Dung Nguyen,Joseph P Tinney,Fangping Yuan,Thomas J Roussel,Ayman El-Baz,Guruprasad Giridharan,Bradley B Keller,Palaniappan Sethu

The American journal of pathology 183:542-57 PubMed23769837

2013

Cells of renin lineage are progenitors of podocytes and parietal epithelial cells in experimental glomerular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey W Pippin,Matthew A Sparks,Sean T Glenn,Sandra Buitrago,Thomas M Coffman,Jeremy S Duffield,Kenneth W Gross,Stuart J Shankland

Molecular vision 17:3156-65 PubMed22171162

2011

Beneficial effects of a novel RAGE inhibitor on early diabetic retinopathy and tactile allodynia.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyuan Li,Jie Tang,Yunpeng Du,Chieh Allen Lee,Timothy S Kern

PloS one 5:e9463 PubMed20209128

2010

Novel siRNA delivery system to target podocytes in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Peter V Hauser,Jeffrey W Pippin,Cora Kaiser,Ronald D Krofft,Paul T Brinkkoetter,Kelly L Hudkins,Dontscho Kerjaschki,Jochen Reiser,Charles E Alpers,Stuart J Shankland

Platelets 19:365-72 PubMed18791943

2008

Flow cytometric measurement of microparticles: pitfalls and protocol modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Mona D Shah,Angela L Bergeron,Jing-Fei Dong,José A López

British journal of haematology 140:465-8 PubMed18217892

2008

AKAP-4: a novel cancer testis antigen for multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Maurizio Chiriva-Internati,Raffaele Ferrari,Yuefei Yu,Cody Hamrick,Nicoletta Gagliano,Fabio Grizzi,Eldo Frezza,Marjorie R Jenkins,Fred Hardwick,Nicholas D'Cunha,W Martin Kast,Everardo Cobos
View all publications
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