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Suitable for IHC-Fr, IHC-P, ICC, ELISA, WB.

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Key facts

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Glucose Oxidase

Applications

IHC-Fr, IHC-P, ICC, ELISA, WB

Clonality

Polyclonal

Reactivity data

Application

IHC-Fr

Reactivity

Reacts

Dilution info

1/10 - 1/100

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/10 - 1/100

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

1/10 - 1/100

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/100 - 1/1000

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/400

Notes

-

Alternative names

Recommended products

Suitable for IHC-Fr, IHC-P, ICC, ELISA, WB.

Key facts

Description

Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Glucose Oxidase

Applications

IHC-Fr, IHC-P, ICC, ELISA, WB

Clonality

Polyclonal

Pre-adsorbed

No

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Purification technique

Affinity purification Immunogen

Purification notes

ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle

Notes

Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.

Preparation of Substrate Solutions:



ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.



Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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