Suitable for IHC-Fr, IHC-P, ICC, ELISA, WB.
Rabbit
Sheep
IgG
Heavy & Light chains
Glucose Oxidase
IHC-Fr, IHC-P, ICC, ELISA, WB
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100 - 1/1000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/400 | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Suitable for IHC-Fr, IHC-P, ICC, ELISA, WB.
Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)
Rabbit
Sheep
IgG
Heavy & Light chains
Glucose Oxidase
IHC-Fr, IHC-P, ICC, ELISA, WB
Polyclonal
No
IgG
Liquid
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Affinity purification Immunogen
ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
Blue Ice
+4°C
+4°C
Avoid freeze / thaw cycle
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.
Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com