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AB99566

Rat monoclonal [11-26] Anti-Mouse IgD delta chain (FITC)

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(2 Publications)

Suitable for Flow Cyt. Cited in 2 publications.

View Alternative Names

Ig delta chain C region secreted form

1 Images
Flow Cytometry - Rat monoclonal [11-26] Anti-Mouse IgD delta chain (FITC) (AB99566)
  • Flow Cyt

Unknown

Flow Cytometry - Rat monoclonal [11-26] Anti-Mouse IgD delta chain (FITC) (AB99566)

BALB/c splenocytes were double-stained with ab99566 and Rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed using a FACScanTM flow cytometer.

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgD

Target specificity

Delta chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Flow Cyt

applications

Clonality

Monoclonal

Clone number

11-26

Isotype

IgG2a

Light chain type

kappa

Specificity

ab99566 reacts with mouse IgD of all haplotypes (e.g., IgDa, IgDb, IgDc), and does not react with other immunoglobulin isotypes. ab99566 does not induce proliferation of mature B cells in vitro, nor does in vivo injection of the monoclonal antibody have any effect on activation of mature B lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg for 10^6 Cells", "notes":"<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

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Target data

See full target information
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Publications (2)

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International immunology 6:355-61 PubMed7514440

1994

Immunofluorescence analysis of B-1 cell ontogeny in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

A M Hamilton,A Lehuen,J F Kearney

Proceedings of the National Academy of Sciences of the United States of America 90:1887-91 PubMed8446604

1993

Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens.

Applications

Unspecified application

Species

Unspecified reactive species

L Nitschke,M H Kosco,G Köhler,M C Lamers
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