AB99574

Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (HRP)

Name: Rat Mono Anti Mouse (IgE epsilon chain) secondary antibody [23G3] HRP (ab99574)
Brand: Abcam Limited
SKU: AB99574
Price: 415 USD
Availability: InStock

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(8 Publications)

Suitable for ICC, ELISA. Ideal for western blot. Cited in 8 publications.

View Alternative Names

Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, IGHE

Unconjugated

Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain
519 FITC

Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (FITC)
Biotin

Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (Biotin)

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgE

Target specificity

Epsilon chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

ELISA, ICC

applications

Clonality

Monoclonal

Clone number

23G3

Isotype

IgG1

Light chain type

kappa

Specificity

ab99574 reacts with the epsilon heavy chain of BALB/c Mouse IgE.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

See full target information IGHE

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6972 PubMed36379950

2022

Mucosal TLR2-activating protein-based vaccination induces potent pulmonary immunity and protection against SARS-CoV-2 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anneliese S Ashhurst,Matt D Johansen,Joshua W C Maxwell,Skye Stockdale,Caroline L Ashley,Anupriya Aggarwal,Rezwan Siddiquee,Stefan Miemczyk,Duc H Nguyen,Joel P Mackay,Claudio Counoupas,Scott N Byrne,Stuart Turville,Megan Steain,James A Triccas,Philip M Hansbro,Richard J Payne,Warwick J Britton

Nature biomedical engineering 6:791-805 PubMed35788687

2022

Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Wang,Kristen D Popowski,Dashuai Zhu,Blanca López de Juan Abad,Xianyun Wang,Mengrui Liu,Halle Lutz,Nicole De Naeyer,C Todd DeMarco,Thomas N Denny,Phuong-Uyen C Dinh,Zhenhua Li,Ke Cheng

Journal of virology 96:e0033622 PubMed35404082

2022

Vesicular Stomatitis Virus-Based Epstein-Barr Virus Vaccines Elicit Strong Protective Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Wei Kong,Xiao Zhang,Guo-Long Bu,Hui-Qin Xu,Yin-Feng Kang,Cong Sun,Qian-Ying Zhu,Run-Bo Ma,Zheng Liu,Yi-Xin Zeng,Mu-Sheng Zeng,Zhu-Long Hu

Frontiers in cell and developmental biology 9:678377 PubMed34169075

2021

Allergen-Specific Treg Cells Upregulated by Lung-Stage Infection Alleviates Allergic Airway Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhidan Li,Wei Zhang,Fang Luo,Jian Li,Wenbin Yang,Bingkuan Zhu,Qunfeng Wu,Xiaoling Wang,Chengsong Sun,Yuxiang Xie,Bin Xu,Zhaojun Wang,Feng Qian,Jiaxu Chen,Yanmin Wan,Wei Hu

Inflammation 42:961-972 PubMed30715691

2019

Chinese Birch Pollen Allergy and Immunotherapy in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijuan Xie,Jia Yin

American journal of translational research 11:280-289 PubMed30787986

2019

Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Kong,Jia Yin

Journal of controlled release : official journal of the Controlled Release Society 293:201-214 PubMed30527753

2018

Liposomes with cyclic RGD peptide motif triggers acute immune response in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Wang,Huan Wang,Kuan Jiang,Yanyu Zhang,Changyou Zhan,Man Ying,Mingfei Zhang,Linwei Lu,Ruifeng Wang,Songli Wang,Diane J Burgess,Hao Wang,Weiyue Lu

Molecular medicine reports 15:3674-3680 PubMed28393183

2017

Anti‑inflammatory effects of dihydromyricetin in a mouse model of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Xu,Shuran Huang,Caiying Wang,Haitao Zhang,Shengcun Fang,Yingming Zhang

View all publications

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Product promise

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Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (HRP)

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Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Clone number

Isotype

Light chain type

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

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