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AB172327

Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647)

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(4 Publications)

Suitable for Flow Cyt, ELISA, IHC-P, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
2 Images
Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)
  • Flow Cyt

Unknown

Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)

Overlay histogram showing subclass specificity of ab172327. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated : ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172327 - Rat monoclonal (SB74g) Anti-Mouse IgG2b, gamma chain - Alexa Fluor® 647) was used at 1/1000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

Alexa Fluor® - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)
  • Alexa Fluor®

Supplier Data

Alexa Fluor® - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)

Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

  • 519 Alexa Fluor® 488

    Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 488)

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgG2b

Target specificity

Gamma chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-P, ELISA, ICC/IF, Flow Cyt

applications

Clonality

Monoclonal

Clone number

SB74g

Isotype

IgG2b

Specificity

Reacts with the y2b heavy chain of mouse IgG2b. Minimal reaction with other mouse immunoglobulins as determined by ELISA. May react with immunoglobulins from other species.

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Complementary Products

Secondary Antibodies

AB172329

Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)

Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (AB172329)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB97255

Goat Anti-Mouse IgG2c heavy chain (HRP)

  • 0
  • 0
Secondary Antibodies

AB97245

Goat Anti-Mouse IgG2a heavy chain (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (AB97245)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000", "notes":"<p><a href='/en-us/products/unavailable/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-alexa-fluorreg-647-ab176105'>ab176105</a> - Mouse monoclonal IgG2b (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Mouse IgG2b
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 14:1127474 PubMed36909232

2023

Delayed denervation-induced muscle atrophy in knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Zhang,Ming Chen,Yi Li,Man Rao,Duanyang Wang,Zhongqi Wang,Licheng Zhang,Pengbin Yin,Peifu Tang

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8321-8337 PubMed34417330

2021

mTOR Signaling Regulates Metabolic Function in Oligodendrocyte Precursor Cells and Promotes Efficient Brain Remyelination in the Cuprizone Model.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa A Jeffries,Lauren E McLane,Luipa Khandker,Marie L Mather,Angelina V Evangelou,Divyangi Kantak,Jennifer N Bourne,Wendy B Macklin,Teresa L Wood

Acta biomaterialia 74:236-246 PubMed29783088

2018

Evaluation of genotoxicity and mutagenic effects of vector/DNA nanocomplexes in transfected mesenchymal stem cells by flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Alireza Nomani,Xuguang Chen,Arash Hatefi

The Journal of neuroscience : the official journal 37:7534-7546 PubMed28694334

2017

Loss of Tuberous Sclerosis Complex1 in Adult Oligodendrocyte Progenitor Cells Enhances Axon Remyelination and Increases Myelin Thickness after a Focal Demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren E McLane,Jennifer N Bourne,Angelina V Evangelou,Luipa Khandker,Wendy B Macklin,Teresa L Wood
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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