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AB172328

Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488)

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(1 Publication)

Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.

View Alternative Names

Ig gamma-3 chain C region

2 Images
Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)
  • Flow Cyt

Unknown

Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)

Overlay histogram showing subclass specificity of ab172328. HeLa cells were fixed with 80% methanol (-20°C, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated : ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172328 - Rat monoclonal (SB76b) Anti-Mouse IgG3 gamma chain - Alexa Fluor® 488) was used at 1/3000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Alexa Fluor® - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)
  • Alexa Fluor®

Supplier Data

Alexa Fluor® - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 488) (AB172328)

Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

  • 665 Alexa Fluor® 647

    Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgG3

Target specificity

Gamma chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Monoclonal

Clone number

SB76b

Isotype

IgG3

Specificity

Reacts with mouse IgG3 as demonstrated by ELISA. Minimal reaction with other mouse isotypes. May react with IgG from other species.

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Complementary Products

Secondary Antibodies

AB172327

Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647)

Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB97245

Goat Anti-Mouse IgG2a heavy chain (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (AB97245)

  • 4
  • 1
Secondary Antibodies

AB97255

Goat Anti-Mouse IgG2c heavy chain (HRP)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Ig gamma-3 chain C region
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 219: PubMed35139155

2022

Single-cell transcriptomic analysis of human colonic macrophages reveals niche-specific subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Domanska,Umair Majid,Victoria T Karlsen,Marianne A Merok,Ann-Christin Røberg Beitnes,Sheraz Yaqub,Espen S Bækkevold,Frode L Jahnsen
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com