Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging.
Rat
Mouse
IgG3
Gamma chain
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
ELISA, IHC-P, ICC/IF, Flow Cyt
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/3000 | Notes - |
Select an associated product type
Ig gamma-3 chain C region
Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging.
Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)
Rat
Mouse
IgG3
Gamma chain
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
ELISA, IHC-P, ICC/IF, Flow Cyt
Monoclonal
SB76b
No
Reacts with mouse IgG3 as demonstrated by ELISA. Minimal reaction with other mouse isotypes. May react with IgG from other species.
IgG3
Liquid
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Overlay histogram showing subclass specificity of ab172329. HeLa cells were fixed with 80% methanol (-20°C, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172329 - Rat monoclonal (SB76b) Anti-Mouse IgG3 gamma chain - Alexa Fluor® 647) was used at 1/3000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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