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Suitable for Flow Cyt, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

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Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (AB99604), expandable thumbnail

Publications

Key facts

Host species
Rat
Target species
Mouse
Target isotype
IgG1
Target specificity
Fc region
Minimal cross-reactivity
Rabbit, Goat, Human
Conjugation
Biotin
Applications
Flow Cyt, ELISA
Clonality
Monoclonal

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/5000.00000 - 1/20000.00000
Notes

-

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Alternative names

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Suitable for Flow Cyt, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Key facts

Description
Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed
Host species
Rat
Target species
Mouse
Target isotype
IgG1
Target specificity
Fc region
Minimal cross-reactivity
Rabbit, Goat, Human
Conjugation
Biotin
Applications
Flow Cyt, ELISA
Clonality
Monoclonal
Clone number
SB77e
Pre-adsorbed
Yes
Specificity

ab99604 reacts with Mouse IgG1 as demonstrated by ELISA. Minimal reaction to Human, Goat and Rabbit IgG. Each batch of Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.

Isotype
IgG2b
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Purification technique
Affinity purification Protein A

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

1 product image

  • Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604), expandable thumbnail

    Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)

    Top: (Negative control) ab99604 staining for Mouse IgG1 in Human blood at 1μg/106 cells.

    Bottom: ab99604 staining in Human blood incubated with mouse anti-Human CD3 (IgG1 isotype) and Streptavidin-FITC.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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