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Suitable for ELISA, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.

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Images

Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (AB99605), expandable thumbnail

Key facts

Host species
Rat
Target species
Mouse
Target isotype
IgG1
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rabbit, Goat, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
ELISA, Flow Cyt, ICC/IF
Clonality
Monoclonal

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
0.1 µg for 106 Cells
Notes

-

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Additional Targets

Alternative names

Recommended products

Suitable for ELISA, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.

Key facts

Description
Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed
Host species
Rat
Target species
Mouse
Target isotype
IgG1
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rabbit, Goat, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
ELISA, Flow Cyt, ICC/IF
Clonality
Monoclonal
Clone number
SB77e
Pre-adsorbed
Yes
Specificity

ab99605 reacts with Mouse IgG1 as demonstrated by ELISA. Each batch of Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.

Isotype
IgG2b
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose

Purification technique
Affinity purification

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (ab99605), expandable thumbnail

    Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (ab99605)

    Top: (Negative control) ab99605 being used against Mouse IgG1 in human blood at 0.1 μg/106 cells.

    Bottom: ab99605 staining human blood incubated with mouse anti-human CD3 (IgG1 isotype) at 0.1 μg/106 cells.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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