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AB99605

Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed

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Suitable for ELISA, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images
Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (AB99605)
  • Flow Cyt

Unknown

Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (AB99605)

Top : (Negative control) ab99605 being used against Mouse IgG1 in human blood at 0.1 μg/106 cells.
Bottom : ab99605 staining human blood incubated with mouse anti-human CD3 (IgG1 isotype) at 0.1 μg/106 cells.

  • Unconjugated

    Rat monoclonal [SB77e] Anti-Mouse IgG1 Fc preadsorbed

  • Alkaline Phosphatase

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed

  • HRP

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed

  • Biotin

    Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed

  • 519 FITC

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgG1

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rabbit, Goat, Human

Pre-adsorbed

Yes

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

ELISA, ICC/IF, Flow Cyt

applications

Clonality

Monoclonal

Clone number

SB77e

Isotype

IgG2b

Specificity

ab99605 reacts with Mouse IgG1 as demonstrated by ELISA. Each batch of Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1 µg for 10^6 Cells", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: Sucrose, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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