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Suitable for Flow Cyt, IHC-Fr. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.


Images

Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (AB172324), expandable thumbnail

Publications

Key facts

Host species
Rat
Target species
Mouse
Target isotype
IgG2a
Target specificity
Heavy chain
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, IHC-Fr
Clonality
Monoclonal

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/2000.00000
Notes

ab171464 - Mouse monoclonal IgG2a (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

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Suitable for Flow Cyt, IHC-Fr. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.

Key facts

Description
Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)
Host species
Rat
Target species
Mouse
Target isotype
IgG2a
Target specificity
Heavy chain
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, IHC-Fr
Clonality
Monoclonal
Clone number
SB84a
Pre-adsorbed
No
Specificity

This antibody is specific to Mouse IgG2a heavy chain

Isotype
IgG1
Light chain type
kappa
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Immunogen

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324), expandable thumbnail

    Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

    Overlay histogram showing subclass specificity of ab172324. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a, heavy chain - Alexa Fluor® 488) was used at 1/1000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com