Suitable for Flow Cyt, IHC-Fr. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 1/1000.00000 - 1/2000.00000 | Notes ab171464 - Mouse monoclonal IgG2a (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody. |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
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Suitable for Flow Cyt, IHC-Fr. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.
This antibody is specific to Mouse IgG2a heavy chain
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
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Overlay histogram showing subclass specificity of ab172324. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a, heavy chain - Alexa Fluor® 488) was used at 1/1000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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