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AB172325

Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 647)

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(4 Publications)

Suitable for Flow Cyt, IHC-Fr. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
2 Images
Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 647) (AB172325)
  • Flow Cyt

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Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 647) (AB172325)

Overlay histogram showing subclass specificity of ab172325. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated : ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172325 - Rat monoclonal (SB84a) Anti-Mouse IgG2a, heavy chain - Alexa Fluor® 647) was used at 1/1000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

Alexa Fluor® - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 647) (AB172325)
  • Alexa Fluor®

Supplier Data

Alexa Fluor® - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 647) (AB172325)

Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

  • 519 Alexa Fluor® 488

    Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgG2a

Target specificity

Heavy chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-Fr, Flow Cyt

applications

Clonality

Monoclonal

Clone number

SB84a

Isotype

IgG1

Light chain type

kappa

Specificity

This antibody is specific to Mouse IgG2a heavy chain

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"<p><a href='/en-us/products/unavailable/mouse-igg2a-kappa-monoclonal-10d7a7b2-isotype-control-alexa-fluorreg-647-ab176104'>ab176104</a> - Mouse monoclonal IgG2a (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.</p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Mouse IgG2a
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical immunology 43:1134-1136 PubMed37099066

2023

Novel Functional Assay to Characterize Mutations in Alternative Pathway of Complement.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria F Gerber,Xiang-Zuo Pan,Howard M Lederman,Tammy M Brady,Robert A Brodsky

Clinical immunology (Orlando, Fla.) 221:108616 PubMed33148511

2020

Ex vivo assays to detect complement activation in complementopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yuan,Jia Yu,Gloria Gerber,Shruti Chaturvedi,Michael Cole,Hang Chen,Ara Metjian,C John Sperati,Evan M Braunstein,Robert A Brodsky

Blood 135:239-251 PubMed31812994

2019

Complement activity and complement regulatory gene mutations are associated with thrombosis in APS and CAPS.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Chaturvedi,Evan M Braunstein,Xuan Yuan,Jia Yu,Alice Alexander,Hang Chen,Eleni Gavriilaki,Ravi Alluri,Michael B Streiff,Michelle Petri,Mark A Crowther,Keith R McCrae,Robert A Brodsky

Transboundary and emerging diseases 67:133-148 PubMed31419374

2019

Model of persistent foot-and-mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Hägglund,Eve Laloy,Katarina Näslund,Florian Pfaff,Michael Eschbaumer,Aurore Romey,Anthony Relmy,Annika Rikberg,Anna Svensson,Helene Huet,Kamila Gorna,Daniela Zühlke,Katharina Riedel,Martin Beer,Stephan Zientara,Labib Bakkali-Kassimi,Sandra Blaise-Boisseau,Jean François Valarcher
View all publications
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Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)

Flow Cytometry - Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (AB172324)

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Secondary Antibodies

AB172327

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Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (AB172327)

  • 0
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AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

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