Anti-rabbit secondary antibodies
Our anti-rabbit secondary antibodies are designed to deliver accurate detection and strong signal amplification in a wide range of immunodetection protocols. They're used when your primary antibody is raised in rabbits and are conjugated to enzymes or fluorophores for clear, enhanced visualization.
Whether you're targeting proteins, antigens, or other molecules in complex samples, these secondary antibodies help ensure your results are both visible and dependable.
High specificity and sensitivity
- Available with a wide range of conjugates, including horseradish peroxidase (HRP), biotin, and various fluorophores such as FITC, Alexa Fluor® dyes, and others.
- This flexibility makes them ideal for multiple applications, such as fluorescence microscopy, western blot, and ELISA, allowing for multiplexing and use in diverse experimental setups.
Quality and consistency
- Produced using stringent quality control measures, ensuring consistent performance batch after batch.
- These antibodies undergo rigorous validation to guarantee high-quality results in your chosen application.
Applications and use cases
Immunohistochemistry (IHC)
- Used in IHC to identify the presence of specific antigens in tissue sections. Fluorophore-conjugated anti-rabbit secondary antibodies are often used for their bright, photostable signals.
Western blot
- Ideal for western blot applications, where they help visualize protein bands on membranes. HRP-conjugated versions are commonly used for enhanced chemiluminescence detection.
Enzyme-linked immunosorbent assay (ELISA)
- Act as critical components in sandwich ELISA setups, aiding in the quantification of antigen amounts using either colorimetric or fluorescence-based readouts.
Flow cytometry
- Employed in flow cytometry to analyze protein expression in cells or cell populations, typically using fluorophore-conjugated forms for rapid, effective signal detection.
FAQ
What fluorophore should I choose for my application?
The choice of fluorophore depends on your detection equipment and the need for multiplexing. For example, FITC is ideal for flow cytometry, while Alexa Fluor® dyes offer a broader range for fluorescence microscopy.
Can these antibodies be used in multiplex assays?
Yes, our anti-rabbit secondary antibodies are available in various conjugates, allowing them to be used in multiplex setups alongside other secondary antibodies targeting different species.
How do I choose between HRP and biotin conjugates?
HRP conjugates are typically used for direct detection methods, providing rapid, sensitive visualization. Biotinylated antibodies, used in conjunction with streptavidin-HRP or other streptavidin conjugates, offer amplification steps that can enhance signal intensity, suitable for detecting low-abundance targets.
Are these secondary antibodies validated for use in both fixed and non-fixed cells?
Yes, our anti-rabbit secondary antibodies have been validated for use in a range of conditions, including both fixed and non-fixed cells, ensuring reliable performance across different experimental conditions.
By using these anti-rabbit secondary antibodies, researchers can enhance their immunodetection protocols, achieving clearer, more accurate results in their studies of complex biological samples.