Anti-rat secondary antibodies
Our anti-rat secondary antibodies are designed to bind specifically to rat-derived primary antibodies, helping amplify signals and improve detection in a range of immunodetection methods.
They’re widely used in a wide range of applications such as western blot, IHC, and ELISA.
We offer a broad selection of conjugates and host species to suit different experimental needs. Each antibody is crafted to deliver high specificity and sensitivity, so you can trust your results, even in complex samples.
Features and specifications
Host species variety
Our anti-rat secondary antibodies are sourced from multiple host species, including goat, donkey, and rabbit, providing flexibility in choosing the appropriate secondary antibody to avoid cross-reactivity in multi-species experiments.
Conjugation options
Available with a variety of labels such as horseradish peroxidase (HRP), biotin, and fluorescent dyes (e.g., Alexa Fluor®, FITC, and PE), facilitating use in different detection systems.
Purity and performance
Antibodies are highly purified and validated for specific performance, ensuring consistent, reliable results across a range of applications.
Formulations
Provided in various formulations, including buffered aqueous solutions, to maintain stability and activity.
Benefits
- High sensitivity and specificity: Ensures that the detection of rat-derived primary antibodies is accurate, minimizing background noise and false positives.
- Versatility: Suitable for a broad array of immunoassays, supporting multifaceted research needs.
- Ease of use: Pre-optimized conjugates and ready-to-use formats simplify experimental workflows and decrease time to results.
Applications and use cases
Immunohistochemistry (IHC)
- Localization of antigens in tissue sections using rat primary antibodies, with visual enhancement provided by our fluorescent or enzyme-conjugated secondary antibodies.
Western blot
- Detection of proteins separated by gel electrophoresis using rat primary antibodies.
Enzyme-linked immunosorbent assay (ELISA)
- Quantification of antigens in a plate-based assay format, where the anti-rat secondary antibodies provide necessary signal amplification.
Immunofluorescence
- Analysis of cells or tissue sections where multiple labeling is required; using different hosts for secondary antibodies prevents cross-reactivity.
FAQ
What should I consider when selecting an anti-rat secondary antibody?
Consider the host species of the primary antibody, the required detection system (e.g., colorimetric, fluorescent), and whether multiple labeling in the same sample is needed. Selecting a secondary antibody from a different host species than the sample material can help to reduce background.
Can I use anti-rat secondary antibodies in multiplex assays?
Yes, our secondary antibodies can be used in multiplex settings, provided that they are conjugated with different labels and that primary antibodies from other species or from the same species with different isotypes are used.
How should I store my anti-rat secondary antibodies?
Most anti-rat secondary antibodies should be stored at 4°C for short-term use or aliquoted and frozen at -20°C for long-term storage to prevent degradation.