AB136791
Sheep F(ab) Anti-Guinea pig IgG H&L (HRP)
Be the first to review this product! Submit a review
|
(0 Publication)
Suitable for ICC, IHC-P, IHC-Fr, WB, ELISA. Ideal for western blot.
Complementary Products
Primary Antibodies
AB9485
Anti-GAPDH antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 88
Primary Antibodies
AB8227
Anti-beta Actin antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 104
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p>DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.</p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p>DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p>4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H<sub>2</sub>O<sub>2</sub>. Incubate 10 min at room temperature.</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p>OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H<sub>2</sub>SO<sub>4</sub>. Reading at 492nm</p>" } } }
Product details
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Very stringent purification protocol.
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 49% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Target data
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com