AB136791
Sheep F(ab) Anti-Guinea pig IgG H&L (HRP)
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Suitable for ICC, IHC-P, IHC-Fr, WB, ELISA. Ideal for western blot.
View Alternative Names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p>DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.</p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p>DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p>4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H<sub>2</sub>O<sub>2</sub>. Incubate 10 min at room temperature.</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p>OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H<sub>2</sub>O<sub>2</sub> (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H<sub>2</sub>SO<sub>4</sub>. Reading at 492nm</p>" } } }
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Very stringent purification protocol.
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 49% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
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Target data
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