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AB96926

Sheep Anti-Rabbit IgG H&L (DyLight® 650)

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(3 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 3 publications.

Key facts

Host species

Sheep

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p>DyLight<sup>®</sup> 650 replaces DyLight<sup>®</sup> 649. The DyLight<sup>®</sup> 650 excitation is 652nm and the emission is 672nm. DyLight<sup>®</sup> 650 hence provides the same far-red fluorescence and photostability as the DyLight<sup>®</sup> 649 dye. Please refer to the vial in order to check whether the product you received is DyLight<sup>®</sup> 649 or DyLight<sup>®</sup> 650. This information however will have no impact on your experiments.</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }

Product details

DyLight® 650 replaces DyLight® 649.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ACS infectious diseases 9:1064-1077 PubMed37053583

2023

Sphingosine Kinases Promote Ebola Virus Infection and Can Be Targeted to Inhibit Filoviruses, Coronaviruses, and Arenaviruses Using Late Endocytic Trafficking to Enter Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Corina M Stewart,Yuxia Bo,Kathy Fu,Mable Chan,Robert Kozak,Kim Yang-Ping Apperley,Geneviève Laroche,Redaet Daniel,André M Beauchemin,Gary Kobinger,Darwyn Kobasa,Marceline Côté

PLoS pathogens 17:e1009275 PubMed33513206

2021

Ebola virus triggers receptor tyrosine kinase-dependent signaling to promote the delivery of viral particles to entry-conducive intracellular compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Corina M Stewart,Alexandra Phan,Yuxia Bo,Nicholas D LeBlond,Tyler K T Smith,Geneviève Laroche,Patrick M Giguère,Morgan D Fullerton,Martin Pelchat,Darwyn Kobasa,Marceline Côté

Nature immunology 16:406-14 PubMed25706747

2015

Proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident CD8⁺ T cells responding to infection.

Applications

IHC

Species

Unspecified reactive species

Tessa Bergsbaken,Michael J Bevan
View all publications

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