JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

5

(3 Reviews)

|

(16 Publications)

10-245 kDa prestained protein ladder (ab116028) is a ready- to-use, three-color protein standard with 12 pre-stained proteins ranging from molecular weights 10 to 245 kDa.

- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
1 Images
SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)
  • SDS-PAGE

Unknown

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

Gel 1 : Tris-Glycine (~4-20%), Gel 2 : Bis-Tris (10%) MOPS buffer, Gel 3 : Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

Key facts

Applications

SDS-PAGE, WB

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris citrate/phosphate, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Urea

storage-buffer

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

  • 5
  • 2
Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Reagents, Controls & Accessories

AB156034

10X RIPA Buffer

SDS-PAGE - 10X RIPA Buffer (AB156034)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>~1.5-2.5 µl per well for general western transferring. Apply more for thicker (&gt; 1.5 mm) or larger gel.</p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.</p>" } } }
style
left-column

Product details

Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

Key product features:

  • Broad range: 10-245 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~1.5 - 2.5μl

Review other protein ladders in the unstained and prestained protein ladder guide.

This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 10: PubMed33120979

2020

Manuka Honey Induces Apoptosis of Epithelial Cancer Cells through Aquaporin-3 and Calcium Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Martinotti,Giorgia Pellavio,Mauro Patrone,Umberto Laforenza,Elia Ranzato

Malaria journal 19:367 PubMed33054833

2020

Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India.

Applications

Unspecified application

Species

Unspecified reactive species

Sharmistha Ghoshal,Pramita Chowdhury,Sanhita Ray,Mitashree Mitra,Sumana Datta Kanjilal,Srikanta Sen,Anjan Kr Dasgupta,Sanghamitra Sengupta

Infection, genetics and evolution : journal of mol 85:104418 PubMed32561295

2020

Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Pramita Chowdhury,Sanhita Ray,Ayan Chakraborty,Srikanta Sen,Anjan Kr Dasgupta,Sanghamitra Sengupta

BMC molecular and cell biology 21:24 PubMed32245408

2020

PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Bashar M Thejer,Partho P Adhikary,Amandeep Kaur,Sarah L Teakel,Ashleigh Van Oosterum,Ishith Seth,Marina Pajic,Katherine M Hannan,Megan Pavy,Perlita Poh,Jalal A Jazayeri,Thiri Zaw,Dana Pascovici,Marina Ludescher,Michael Pawlak,Juan C Cassano,Lynne Turnbull,Mitra Jazayeri,Alexander C James,Craig P Coorey,Tara L Roberts,Simon J Kinder,Ross D Hannan,Ellis Patrick,Mark P Molloy,Elizabeth J New,Tanja N Fehm,Hans Neubauer,Ewa M Goldys,Leslie A Weston,Michael A Cahill

Antioxidants (Basel, Switzerland) 8: PubMed31817022

2019

The Association of Ascorbic Acid, Deferoxamine and N-Acetylcysteine Improves Cardiac Fibroblast Viability and Cellular Function Associated with Tissue Repair Damaged by Simulated Ischemia/Reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Parra-Flores,Jaime A Riquelme,Paula Valenzuela-Bustamante,Sebastian Leiva-Navarrete,Raúl Vivar,Jossete Cayupi-Vivanco,Esteban Castro,Claudio Espinoza-Pérez,Felipe Ruz-Cortés,Zully Pedrozo,Sergio Lavandero,Ramon Rodrigo,Guillermo Diaz-Araya

Cellular and molecular life sciences : CMLS 77:2235-2253 PubMed31473770

2019

Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca signals and nitric oxide release in human brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sharon Negri,Pawan Faris,Giorgia Pellavio,Laura Botta,Matteo Orgiu,Greta Forcaia,Giulio Sancini,Umberto Laforenza,Francesco Moccia

Investigational new drugs 38:547-557 PubMed31155684

2019

Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Louis Depoix,Arthur Colson,Mina Mhallem-Gziri,Corinne Hubinont,Frederic Debieve

Proceedings of the National Academy of Sciences of the United States of America 116:11997-12006 PubMed31127039

2019

Histone deacetylase 4 promotes type I interferon signaling, restricts DNA viruses, and is degraded via vaccinia virus protein C6.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxu Lu,Jennifer H Stuart,Callum Talbot-Cooper,Shuchi Agrawal-Singh,Brian Huntly,Andrei I Smid,Joseph S Snowden,Liane Dupont,Geoffrey L Smith

Molecules (Basel, Switzerland) 24: PubMed31010117

2019

Propolis Induces AQP3 Expression: A Possible Way of Action in Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Martinotti,Giorgia Pellavio,Umberto Laforenza,Elia Ranzato

Cancers 11: PubMed30991693

2019

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Induces Intracellular Ca Release through the Two-Pore Channel TPC1 in Metastatic Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pawan Faris,Giorgia Pellavio,Federica Ferulli,Francesca Di Nezza,Mudhir Shekha,Dmitry Lim,Marcello Maestri,Germano Guerra,Luigi Ambrosone,Paolo Pedrazzoli,Umberto Laforenza,Daniela Montagna,Francesco Moccia
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com