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Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa.


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SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (AB116028), expandable thumbnail

Publications

Key facts

Applications

WB, SDS-PAGE

Form

Liquid

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

~1.5-2.5 µl per well for general western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

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Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa.

Key facts

Applications

WB, SDS-PAGE

Form

Liquid

Storage buffer

pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris citrate/phosphate, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Urea

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

Key product features:

  • Broad range: 10-245 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~1.5 - 2.5μl

Review other protein ladders in the unstained and prestained protein ladder guide.

This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028), expandable thumbnail

    SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

    Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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