JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

Be the first to review this product! Submit a review

|

(0 Publication)

Prestained Protein Ladder ab3259945 is a combination of 11 pre-stained proteins covering a range of MW from 11-250 kDa.
1 Images
SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Key facts

Applications

SDS-PAGE, WB

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 27.9% Glycerol (glycerin, glycerine), 25% Urea, 14.7% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2.7% Sodium lauryl sulfate, 2% Tris phosphate

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.</p>" } } }

Product details

Prestained Protein Ladder ab3259945 is a combination of 11 pre-stained proteins covering a range of MW from 11-250 kDa. It is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key product features:

  • Broad range: 11-180 kDa
  • Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
  • Easily identified: Proteins are covalently coupled with a blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa, and a purple band at 250 kDa serve as reference bands.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Review other protein ladders in the unstained and prestained protein ladder guide.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

Target data

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com