Prestained Protein Ladder AB313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
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Key facts
- Applications
- SDS-PAGE, WB
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
Application WB | Reactivity Reacts | Dilution info - | Notes For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl. |
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Prestained Protein Ladder AB313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Key facts
- Applications
- SDS-PAGE, WB
- Form
- Liquid
- Storage buffer
pH: 7 - 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 25% Urea, 3% Sodium lauryl sulfate, 2% Tris phosphate, 0.016% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Prestained Protein Ladder ab313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Key Product Features: Extra broad molecular weight (3-260 kDa).
Ready to use: The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (ab313996)
Guide for Molecular Weight Estimation (kDa)
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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