Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)
5
(1 Review)
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(3 Publications)
- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
- SDS-PAGE
Supplier Data
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)
Gel 1 : Tris-Glycine (4-15%) SDS buffer; Gel 2 : Bis-Tris (4-12%) MOPS buffer; Gel 3 : Bis-Tris (4-12%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
- SDS-PAGE
Unknown
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)
Gel 1 : Tris-Glycine (~4-20%), Gel 2 : Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
Complementary Products
Reagents, Controls & Accessories
AB116028
Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)
- 5
- 3
Reactivity data
Product details
Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).
This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 3.5-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 - 2.5μl
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Target data
Publications (3)
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Frontiers in cell and developmental biology 8:611503 PubMed33553147
2021
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International journal of molecular sciences 20: PubMed31810173
2019
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Cells 8: PubMed31635340
2019
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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