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Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa.


Images

Key facts

Applications

SDS-PAGE, WB

Form

Liquid

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

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Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa.

Key facts

Applications

SDS-PAGE, WB

Form

Liquid

Storage buffer

pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris citrate/phosphate, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Urea

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).

This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Key product features:

  • Broad range: 3.5-245 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~1.5 - 2.5μl

Review other protein ladders in the unstained and prestained protein ladder guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029), expandable thumbnail

    SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)

    Gel 1: Tris-Glycine (4-15%) SDS buffer; Gel 2: Bis-Tris (4-12%) MOPS buffer; Gel 3: Bis-Tris (4-12%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

  • SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029), expandable thumbnail

    SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)

    Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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