Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa.
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- Frontiers in cell and developmental biology 8:6115032021SHP2 Nuclear/Cytoplasmic Trafficking in Granulosa Cells Is Essential for Oocyte Meiotic Resumption and Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Idrees,Vikas Kumar,Myeong-Don Joo,Niaz Ali,Keun-Woo Lee,Il-Keun KongPubMed 33553147
- International journal of molecular sciences 20:2019The PPARδ Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Idrees et. al.PubMed 31810173
- Cells 8:2019PTPN11 (SHP2) Is Indispensable for Growth Factors and Cytokine Signal Transduction During Bovine Oocyte Maturation and Blastocyst Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Idrees et. al.PubMed 31635340
Key facts
- Applications
SDS-PAGE, WB
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
Application WB | Reactivity Reacts | Dilution info - | Notes ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel. |
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Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa.
Key facts
- Applications
SDS-PAGE, WB
- Form
Liquid
- Storage buffer
pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris citrate/phosphate, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Urea
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).
This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 3.5-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 - 2.5μl
Review other protein ladders in the unstained and prestained protein ladder guide.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)
Gel 1: Tris-Glycine (4-15%) SDS buffer; Gel 2: Bis-Tris (4-12%) MOPS buffer; Gel 3: Bis-Tris (4-12%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)
Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
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