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Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa.

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SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592), expandable thumbnail

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Key facts

Applications
SDS-PAGE, WB
Form
Liquid

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively.

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl.

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Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa.

Key facts

Applications
SDS-PAGE, WB
Form
Liquid
Storage buffer

pH: 7.5
Preservative: 0.01% Sodium azide
Constituents: 33% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 1.37% Tris phosphate, 0.15% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key product features:

  • Broad range: 6.5-270 kDa
  • Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
  • Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Review other protein ladders in the unstained and prestained protein ladder guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (ab234592), expandable thumbnail

    SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (ab234592)

    SDS-PAGE analysis with ab234592.

    Gel 1: Tris-Glycine 4-20%

    Gel 2: Bis-Tris 4-12% MES buffer.

    The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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