Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
SDS-PAGE, WB
Liquid
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
Application WB | Reactivity Reacts | Dilution info - | Notes ~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel. |
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Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
SDS-PAGE, WB
Liquid
pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2% Sodium lauryl sulfate, 1% Tris phosphate, 0.02% Urea
Blue Ice
1-2 weeks
+4°C
-20°C
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
Review other protein ladders in the unstained and prestained protein ladder guide.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Gel 1: Tris-Glycine (~4-20%) Gel 2: Bis-Tris (12%) MOPS buffer Gel 3: Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5μl per well.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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