Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
5
(2 Reviews)
|
(5 Publications)
- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essential range western blot workflow
- SDS-PAGE
Unknown
SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)
Gel 1 : Tris-Glycine (~4-20%) Gel 2 : Bis-Tris (12%) MOPS buffer Gel 3 : Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
- WB
AbReview47271****
Western blot - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)
Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5μl per well.
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Reactivity data
Product details
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 10-180 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~2 - 3 μl for western blots
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Target data
Publications (5)
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BMC infectious diseases 21:300 PubMed33761869
2021
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Saudi journal of biological sciences 28:891-900 PubMed33424381
2021
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Journal of cell communication and signaling 15:107-123 PubMed33398722
2021
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Cell & bioscience 7:71 PubMed29270287
2017
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Journal of cell science 127:124-36 PubMed24144701
2013
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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