Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
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Publications
- BMC infectious diseases 21:3002021Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZahra Shadman,Safar Farajnia,Mohammad Pazhang,Mohammadreza Tohidkia,Leila Rahbarnia,Saeed Najavand,Sayna TorabyPubMed 33761869
- Saudi journal of biological sciences 28:891-9002021Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMukhambetkali Burkitbayev,Nadezhda Bachilova,Meruyert Kurmanbayeva,Karakoz Tolenova,Nurgul Yerezhepova,Moldir Zhumagul,Asem Mamurova,Bexultan Turysbek,Gulim DemeuPubMed 33424381
Key facts
- Applications
SDS-PAGE, WB
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
Application WB | Reactivity Reacts | Dilution info - | Notes ~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel. |
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Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
Key facts
- Applications
SDS-PAGE, WB
- Form
Liquid
- Storage buffer
pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2% Sodium lauryl sulfate, 1% Tris phosphate, 0.02% Urea
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 10-180 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~2 - 3 μl for western blots
Review other protein ladders in the unstained and prestained protein ladder guide.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
Gel 1: Tris-Glycine (~4-20%) Gel 2: Bis-Tris (12%) MOPS buffer Gel 3: Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
This image is courtesy of an anonymous customer review.Western blot - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5μl per well.
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Product protocols
- Visit the general protocols
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