Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
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- SDS-PAGE
Supplier Data
SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)
SDS-PAGE analysis with ab234617.
Gel 1 : Tris-Glycine 10%
Gel 2 : Bis-Tris 4-12% MOPS buffer
Gel 3 : Bis-Tris 4-12% MES buffer.
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
Reactivity data
Product details
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 10-180 kDa
- Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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