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AB234618

Unstained Protein Ladder (10 - 200 kDa)

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Unstained Protein Ladder (ab234618) is a ready-to-use protein standard with 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.

- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-20%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
1 Images
SDS-PAGE - Unstained Protein Ladder (10 - 200 kDa) (AB234618)
  • SDS-PAGE

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SDS-PAGE - Unstained Protein Ladder (10 - 200 kDa) (AB234618)

Key facts

Applications

WB, SDS-PAGE

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 21.6% Urea, 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris phosphate, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 µl per loading for 15-well or 10-well mini-gel.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 µl.</p>" } } }

Product details

Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.

This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key features include:

  • Broad range: 10-200 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easily identified: The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25 kDa and 85 kDa protein bands serving as the two reference bands.

5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for Western transferring.

Learn more about our unstained and prestained protein ladders in our guide.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

Target data

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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