AB110338
Bovine Heart Mitochondria suitable for WB, SDS-PAGE, IP. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Reactivity data
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Product details
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 μl of the mitochondrial membranes supplied be solubilized by the addition of 20 μl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.
1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 μl of the mitochondrial membranes supplied be solubilized by the addition of 20 μl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.
Properties and storage information
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Publications (3)
Recent publications for all applications. Explore the full list and refine your search
Scientific reports 8:8548 PubMed29867098
2018
Mitochondrial function is impaired in the skeletal muscle of pre-frail elderly.
Applications
Unspecified application
Species
Unspecified reactive species
Pénélope A Andreux,Marcus P J van Diemen,Maxime R Heezen,Johan Auwerx,Chris Rinsch,Geert Jan Groeneveld,Anurag Singh
Redox biology 11:415-428 PubMed28064117
2017
Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes.
Applications
FuncS
Species
Human
María Gómez-Serrano,Emilio Camafeita,Juan A López,Miguel A Rubio,Irene Bretón,Inés García-Consuegra,Eva García-Santos,Jesús Lago,Andrés Sánchez-Pernaute,Antonio Torres,Jesús Vázquez,Belén Peral
Biochemistry 51:2258-64 PubMed22385295
2012
Proteolytic regulation of the mitochondrial cAMP-dependent protein kinase.
Applications
Unspecified application
Species
Unspecified reactive species
Jennifer R Shell,David S Lawrence
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