Hela-DFO treated (0.5mM, 24h) Nuclear Lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- Nature communications 9:42962018Early redox activities modulate Xenopus tail regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernando Ferreira et. al.PubMed 30327466
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
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Hela-DFO treated (0.5mM, 24h) Nuclear Lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Disease
- Adenocarcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
We recommend aliquoting the extracts into single use fractions and then
storing them at -80°C.
The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO).
DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.
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4 product images
Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)
Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)
We recommend using 5% milk in TBST as the blocking agent, decreasing to 2% milk in TBST during primary and secondary antibody incubation.
Blots were developed with Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) secondary antibody
All lanes: Western blot - Anti-HIF-1 alpha antibody [H1alpha67] (Anti-HIF-1 alpha antibody [H1alpha67] ab1) at 5 µg/mL
Lane 1: Western blot - HeLa nuclear extract lysate (HeLa nuclear extract lysate ab150036) at 40 µg
Lane 2: Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880) at 40 µg
Lane 3: HeLa nuclear control at 40 µg
Lane 4: HeLa nuclear DFO treated at 40 µg
Lane 5: Western blot - Recombinant Human HIF-1 alpha protein (Recombinant Human HIF-1 alpha protein ab154478) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 20min
Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)
Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)
Abcam recommends using 5% milk as the blocking agent, decreasing to 2% milk during primary and secondary incubation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-HIF-1 alpha antibody [EP1215Y] (Anti-HIF-1 alpha antibody [EP1215Y] ab51608) at 1/2000 dilution
Lane 1: Western blot - HeLa nuclear extract lysate (HeLa nuclear extract lysate ab150036) at 40 µg
Lane 2: Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880) at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 110 kDa
Exposure time: 8min
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