HeLa nuclear extract lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- International journal of cancer 144:615-6302018DNA primase polypeptide 1 (PRIM1) involves in estrogen-induced breast cancer formation through activation of the G2/M cell cycle checkpoint.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Hwa Lee,Li-Ching Chen,Chia-Jung Lee,Chi-Cheng Huang,Yuan-Soon Ho,Po-Sheng Yang,Chi-Tang Ho,Hang-Lung Chang,I-Hsuan Lin,Hui-Wen Chang,Yun-Ru Liu,Chih-Hsiung Wu,Shih-Hsin TuPubMed 30097999
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
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HeLa nuclear extract lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Disease
- Adenocarcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
We recommend aliquoting the extracts into single use fractions and then
storing them at -80°C.
This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
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8 product images
SDS-PAGE - HeLa nuclear extract lysate (ab150036)
All lanes stained with Optiblot Blue (InstantBlue® Coomassie Protein Stain (ISB1L) ab119211) (20 ml)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
Lysates/proteins at 10 μg per lane.
Western blot - HeLa nuclear extract lysate (ab150036)
Western blot - HeLa nuclear extract lysate (ab150036)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197874 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: HRP Anti-TATA binding protein TBP antibody [mAbcam 51841] - Loading Control (ab197874) at 1/5000 dilution
Lane 1: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 20 µg
Lane 2: Western blot - HeLa nuclear extract lysate (ab150036) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1min
Western blot - HeLa nuclear extract lysate (ab150036)
Western blot - HeLa nuclear extract lysate (ab150036)
Western blot - HeLa nuclear extract lysate (ab150036)
Abcam recommends using 5% milk as the blocking agent, decreasing to 2% milk during primary and secondary incubation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-HIF-1 alpha antibody [EP1215Y] (Anti-HIF-1 alpha antibody [EP1215Y] ab51608) at 1/2000 dilution
Lane 1: Western blot - HeLa nuclear extract lysate (ab150036) at 40 µg
Lane 2: Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (Hela-DFO treated (0.5mM, 24h) Nuclear Lysate ab180880) at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 110 kDa
Exposure time: 8min
Western blot - HeLa nuclear extract lysate (ab150036)
Western blot - HeLa nuclear extract lysate (ab150036)
We recommend using 5% milk in TBST as the blocking agent, decreasing to 2% milk in TBST during primary and secondary antibody incubation.
Blots were developed with Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) secondary antibody
All lanes: Western blot - Anti-HIF-1 alpha antibody [H1alpha67] (Anti-HIF-1 alpha antibody [H1alpha67] ab1) at 5 µg/mL
Lane 1: Western blot - HeLa nuclear extract lysate (ab150036) at 40 µg
Lane 2: Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (Hela-DFO treated (0.5mM, 24h) Nuclear Lysate ab180880) at 40 µg
Lane 3: HeLa nuclear control at 40 µg
Lane 4: HeLa nuclear DFO treated at 40 µg
Lane 5: Western blot - Recombinant Human HIF-1 alpha protein (Recombinant Human HIF-1 alpha protein ab154478) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 20min
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