HeLa nuclear extract lysate (TNF alpha stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
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HeLa nuclear extract lysate (TNF alpha stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Disease
- Adenocarcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The HeLa nuclear extracts have been collected in Lysis Buffer after a
30 minute incubation with or without TNF alpha.
The protein content has been determined by a Bradford based assay (20 ng/ml).
We recommend aliquoting the extracts into single use fractions and then
storing.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)
Each lot has been tested for NFkappaB activation. The signal intensity for NFkappaB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.
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