HeLa nuclear extract lysate (TPA stimulated 2 hr) suitable for EMSA, WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
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HeLa nuclear extract lysate (TPA stimulated 2 hr) suitable for EMSA, WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Disease
- Adenocarcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting.
We recommend aliquoting the extracts into single use fractions and then
storing.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)
All lanes: Western blot - Anti-Egr1 antibody [mAbcam55160] (Anti-Egr1 antibody [mAbcam55160] ab55160) at 5 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653) at 20 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55 kDa, 85 kDa
Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (Anti-Egr1 antibody [mAbcam55160] ab55160).
All lanes: Western blot - Anti-Egr1 antibody [mAbcam55160] - BSA and Azide free (Anti-Egr1 antibody [mAbcam55160] - BSA and Azide free ab264527) at 5 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653) at 20 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55 kDa, 85 kDa
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