Hep G2 nuclear extract lysate suitable for EMSA, WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Images
Publications
Filter by
- American journal of human genetics 108:411-4302021Integrative analysis of liver-specific non-coding regulatory SNPs associated with the risk of coronary artery disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlakya Selvarajan,Anu Toropainen,Kristina M Garske,Maykel López Rodríguez,Arthur Ko,Zong Miao,Dorota Kaminska,Kadri Õunap,Tiit Örd,Aarthi Ravindran,Oscar H Liu,Pierre R Moreau,Ashik Jawahar Deen,Ville Männistö,Calvin Pan,Anna-Liisa Levonen,Aldons J Lusis,Sami Heikkinen,Casey E Romanoski,Jussi Pihlajamäki,Päivi Pajukanta,Minna U KaikkonenPubMed 33626337
Key facts
- Cell type
- Hep G2
- Species or organism
- Human
- Tissue
- Liver
- Form
- Liquid
Associated Products
Select an associated product type
3 products for Alternative Product
Recommended products
Hep G2 nuclear extract lysate suitable for EMSA, WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- Hep G2
- Species or organism
- Human
- Tissue
- Liver
- Form
- Liquid
- Disease
- Hepatocellular Carcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cell line: HepG2 (Human hepatocellular carcinoma).
We recommend aliquoting the extracts into single use fractions and then storing them.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
13 product images
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-BRG1 antibody (Anti-BRG1 antibody ab4081) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat nuclear extract lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 235 kDa, 40 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-cbx7 antibody (Anti-cbx7 antibody ab21873) at 1 µg
Lane 1: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 2: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 3: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 100 kDa, 34 kDa, 70 kDa
Exposure time: 30s
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-TDP43 antibody (Anti-TDP43 antibody ab41881) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
Lane 7: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 8: K-562 (Human) Nuclear Lysate - tumor cell line (ab29309) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 47 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-cbx7 antibody (Anti-cbx7 antibody ab21873) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 100 kDa, 34 kDa, 70 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade ab63766) at 1 µg/mL
Lane 1: Testis (Mouse) Tissue Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Testis (Rat) Tissue Lysate at 10 µg
Lane 4: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa, 45 kDa, 55 kDa
Exposure time: 3min
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-CDCA2 antibody (Anti-CDCA2 antibody ab45129) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 125 kDa, 40 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-FOXA2 antibody (Anti-FOXA2 antibody ab23630) at 1 µg/mL
Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 140 kDa, 52 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-JDP2 antibody (Anti-JDP2 antibody ab40916) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 5: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
Lane 6: Du145 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 19 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
Anti-CBP80 antibody ab42389 detects a band at 80 kDa which corresponds to the NCBP 80 kDa subunit.
All lanes: Western blot - Anti-CBP80 antibody (Anti-CBP80 antibody ab42389) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4: Jurkat nuclear extract lysate (ab14844) at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
Lane 7: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 80 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-PROX1 antibody [5G10] (Anti-PROX1 antibody [5G10] ab33219) at 10 µg/mL
Lane 1: Western blot - Hep G2 nuclear extract lysate (ab14660) at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 20min
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Western blot - Anti-CtIP antibody (Anti-CtIP antibody ab38016) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 101 kDa
Observed band size: 110 kDa, 25 kDa, 250 kDa
Western blot - Hep G2 nuclear extract lysate (ab14660)
All lanes: Anti-MDC1 (phospho T4) antibody (ab35967) at 1 µg/mL
Lanes 1 and 4: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 2 and 5: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
Lanes 3 and 6: Hep G2 (Human) Nuclear Lysate - IFN gamma treated (ab14662) at 10 µg
Lane 7: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg with Human MDC1 (unmodified ) peptide (ab36514)
Lane 8: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg with Human MDC1 (unmodified ) peptide (ab36514)
Lane 9: Hep G2 (Human) Nuclear Lysate - IFN gamma treated (ab14662) at 10 µg with Human MDC1 (unmodified ) peptide (ab36514)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Western blot - Hep G2 nuclear extract lysate (ab14660)
Thrombin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-Thrombin antibody [5G9] (Anti-Thrombin antibody [5G9] ab17199) at 1 µg/mL
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 2: Human Plasma Total Protein Lysate at 10 µg
Lane 3: Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 40 kDa, 75 kDa
Exposure time: 12min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com