AB14862
MCF7 nuclear extract lysate (H2O2 treated)
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MCF7 nuclear extract lysate (H2O2 treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (AB14862)
Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.
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Product details
The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide. The protein content has been determined by a Bradford-based assay.
Properties and storage information
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
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