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MCF7 nuclear extract lysate (H2O2 treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (AB14862), expandable thumbnail

Key facts

Cell type
MCF7
Species or organism
Human
Tissue
Breast
Form
Liquid

Target data

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MCF7 nuclear extract lysate (H2O2 treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Cell type
MCF7
Form
Liquid
Disease
Adenocarcinoma
Concentration
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Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide.
The protein content has been determined by a Bradford-based assay.

Product promise

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Terms & Conditions.

1 product image

  • Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (ab14862), expandable thumbnail

    Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (ab14862)

    Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 μg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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