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Mouse brain tissue lysate - total protein (0 days) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188), expandable thumbnail
  • Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188), expandable thumbnail
  • Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188), expandable thumbnail
  • Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188), expandable thumbnail
  • Western blot - Mouse brain tissue lysate - total protein (0 days) (AB7188), expandable thumbnail

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Mouse brain tissue lysate - total protein (0 days) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Liquid
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Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
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Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Source: Outbred NIH-Swiss Albino mice

The mouse brain tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

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17 product images

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-Neuronatin antibody (Anti-Neuronatin antibody ab27266) at 1 µg/mL

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-Doublecortin antibody (Anti-Doublecortin antibody ab18723) at 1 µg/mL

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 45 kDa, 49 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-BLBP antibody (Anti-BLBP antibody ab32423) at 1/250 dilution

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 15 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Rabbit polyclonal to Lingo1 (Anti-Lingo1 antibody ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)

    All lanes: Western blot - Anti-Lingo1 antibody (Anti-Lingo1 antibody ab23631) at 1 µg/mL

    Lane 1: Mouse brain at 20 µg

    Lane 2: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Lane 3: Human Brain Tissue Lysate at 20 µg

    Secondary

    All lanes: Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 70 kDa

    Observed band size: 17 kDa, 34 kDa, 83 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-Hippocalcin antibody (Anti-Hippocalcin antibody ab24560) at 1 µg/mL

    Lane 1: Human brain tissue lysate - total protein (ab29466) at 20 µg

    Lane 2: Brain (Rat) Tissue Lysate at 20 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 20 µg

    Lane 4: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 14 kDa, 23 kDa, 42 kDa, 52 kDa

    Exposure time: 4min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    All lanes: Western blot - Anti-Plexin A2 antibody (Anti-Plexin A2 antibody ab39357) at 2 µg/mL

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary

    All lanes: Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 211 kDa

    Observed band size: 230 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-Neuronatin antibody (Anti-Neuronatin antibody ab27266) at 1 µg/mL

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa

    Observed band size: 10 kDa, 70-75 kDa

    Exposure time: 5min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with Anti-Plexin A4 antibody ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Plexin A4 antibody (Anti-Plexin A4 antibody ab39350) at 1 µg/mL

    Lane 1: Brain (Mouse) Tissue Lysate at 20 µg

    Lane 2: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Lane 3: Brain (Rat) Tissue Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/35000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 212 kDa

    Observed band size: 190 kDa, 213 kDa, 41 kDa, 70 kDa

    Exposure time: 8min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    We are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.

    All lanes: Western blot - Anti-Plexin A4 antibody (Anti-Plexin A4 antibody ab39350) at 1/250 dilution

    Lane 1: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Lane 3: Dorsal Root Ganglia (Mouse) Tissue Lysate at 10 µg

    Lane 4: Brain (Mouse) Tissue Lysate at 10 µg with Mouse Plexin A4 peptide (ab39349)

    Lane 5: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg with Mouse Plexin A4 peptide (ab39349)

    Lane 6: Dorsal Root Ganglia (Mouse) Tissue Lysate at 10 µg with Mouse Plexin A4 peptide (ab39349)

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 212 kDa

    Observed band size: 190 kDa, 213 kDa, 63 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-SNAP25 antibody (Anti-SNAP25 antibody ab41726) at 1 µg/mL

    Lane 1: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 2: Spinal Cord (Rat) Tissue lysate at 10 µg

    Lane 3: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 26 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Lane 1: Marker

    Lanes 2 - 4: Western blot - Anti-beta Arrestin 1 antibody (Anti-beta Arrestin 1 antibody ab31868) at 1 µg/mL

    Lane 2: Mouse Brain at 20 µg

    Lane 3: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Lane 4: Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

    Secondary

    All lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 100 kDa, 50 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-DIO2 antibody (Anti-DIO2 antibody ab77779) at 1 µg/mL

    Lane 1: Mouse Retina Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Lane 3: Brain (Rat) Tissue Lysate at 10 µg

    Lane 4: Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary

    All lanes: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa

    Observed band size: 35 kDa, 40 kDa, 55 kDa

    Exposure time: 3min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-Torsin A/DYT1 antibody (Anti-Torsin A/DYT1 antibody ab34540) at 1 µg/mL

    Lane 1: Marker

    Lane 2: Mouse Brain at 10 µg

    Lane 3: Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

    Lane 4: Mouse Heart Mouse Tissue Lysate at 10 µg

    Lane 5: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 6: Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg

    Lane 7: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Lane 8: Mouse Embryonic Fibroblast 1 at 10 µg

    Lane 9: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa

    Observed band size: 105 kDa, 38 kDa, 65 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-SATB2 antibody (Anti-SATB2 antibody ab34735) at 1 µg/mL

    All lanes: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 30 kDa, 85 kDa

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Anti-GluN2D antibody ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

    All lanes: Western blot - Anti-GluN2D antibody (Anti-GluN2D antibody ab35448) at 1 µg/mL

    Lane 1: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Lane 2: Brain (Mouse) Tissue Lysate, 7 days old at 20 µg

    Lane 3: Brain (Rat) Tissue Lysate, 0 Days old at 20 µg

    Lane 4: Brain (Rat) Tissue Lysate, 7 days old at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 144 kDa

    Observed band size: 125 kDa, 143 kDa, 45 kDa, 60 kDa, ~200 kDa

    Exposure time: 20min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    LRRTM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    All lanes: Western blot - Anti-LRRTM1 antibody (Anti-LRRTM1 antibody ab106414) at 1/250 dilution

    Lane 1: Forebrain (Mouse) Tissue Lysate at 10 µg

    Lane 2: Mouse Hippocampus Tissue Lysate at 10 µg

    Lane 3: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Lane 4: Mouse Olfactory Bulb Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa

    Observed band size: 130 kDa, 30 kDa, 40 kDa, 65 kDa

    Exposure time: 8min

  • Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188), expandable thumbnail

    Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)

    All lanes: Western blot - Anti-CAPS1 antibody (Anti-CAPS1 antibody ab32011) at 1 µg/mL

    Lane 1: Brain (Rat) Tissue Lysate at 10 µg

    Lane 2: Brain (Rat) Tissue Lysate (ab7942)Brain (Rat) Tissue Lysate (ab7942) at 10 µg

    Lane 3: Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Lane 3: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 4: Mouse Fetus (14 Day Old) Tissue Lysate (ab46769)Mouse Fetus (14 Day Old) Tissue Lysate (ab46769) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

    Lane 5: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

    Lane 6: Spinal Cord (Mouse) Tissue Lysate (ab50253)Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg with CAPS1 peptide (ab32785)CAPS1 peptide (ab32785)

    Lane 7: Spinal Cord (Rat) Tissue Lysate (ab50252)Spinal Cord (Rat) Tissue Lysate (ab50252) at 10 µg

    Lane 8: Hippocampus (Mouse) Tissue Lysate (ab50259)Hippocampus (Mouse) Tissue Lysate (ab50259) at 10 µg

    Secondary

    All lanes: Rabbit IgG secondary antibody (ab28446)Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 153 kDa

    Observed band size: 146 kDa, 55 kDa

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