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AB29544

Mouse colon tissue lysate - total protein

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Mouse colon tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Western blot - Mouse colon tissue lysate - total protein (AB29544)
  • WB

Project2186****

Western blot - Mouse colon tissue lysate - total protein (AB29544)

The Ihh protein has a predicted molecular weight of 45 kDa. The first 27 amino acids of the Ihh sequence act as a signal sequence, and when cleaved the protein has an expected molecular weight of 42 kDa.

All lanes:

Western blot - Anti-Ihh antibody (<a href='/en-us/products/primary-antibodies/ihh-antibody-ab39634'>ab39634</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse colon tissue lysate - total protein (ab29544) at 10 µg

Lane 3:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 42 kDa

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Key facts

Form

Liquid

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }
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Product details

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting
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Product protocols

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