Mouse eye tissue lysate - total protein

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• WB

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Western blot - Mouse eye tissue lysate - total protein (AB4029)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab114138 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Otx2 antibody (<a href='/en-us/products/primary-antibodies/otx2-antibody-ab114138'>ab114138</a>) at 1 µg/mL

Lane 1:

E10 Mouse Embryo Brain Tissue Lysate at 10 µg

Lane 2:

E14 Mouse Embryo Brain Tissue Lysate at 10 µg

Lane 3:

E16 Ms Embryo Brain Tissue Lysate at 10 µg

Lane 4:

Western blot - Mouse eye tissue lysate - total protein (ab4029) at 10 µg

Lane 5:

Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

WERI (Human Retinoblastoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 32 kDa

Observed band size: 37 kDa,62 kDa

true

Exposure time: 12min