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Mouse eye tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Western blot - Mouse eye tissue lysate - total protein (AB4029), expandable thumbnail

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Liquid

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Mouse eye tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Liquid
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Source: Outbred NIH-Swiss Albino mice.

Mouse whole eye lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecyl sulfate (SDS), 1 mM sodium ethylene diamine tetra acetate, 1 mM phenyl methyl sulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation (12000rpm). Protein concentration was determined with Bio-Rad protein assay.

The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.

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1 product image

  • Western blot - Mouse eye tissue lysate - total protein (ab4029), expandable thumbnail

    Western blot - Mouse eye tissue lysate - total protein (ab4029)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-Otx2 antibody ab114138 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    All lanes: Western blot - Anti-Otx2 antibody (Anti-Otx2 antibody ab114138) at 1 µg/mL

    Lane 1: E10 Mouse Embryo Brain Tissue Lysate at 10 µg

    Lane 2: E14 Mouse Embryo Brain Tissue Lysate at 10 µg

    Lane 3: E16 Ms Embryo Brain Tissue Lysate at 10 µg

    Lane 4: Western blot - Mouse eye tissue lysate - total protein (ab4029) at 10 µg

    Lane 5: Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg

    Lane 6: WERI (Human Retinoblastoma) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa

    Observed band size: 37 kDa, 62 kDa

    Exposure time: 12min

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