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Mouse lung normal tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Western blot - Mouse lung normal tissue lysate - total protein (AB29297), expandable thumbnail
  • Western blot - Mouse lung normal tissue lysate - total protein (AB29297), expandable thumbnail
  • Western blot - Mouse lung normal tissue lysate - total protein (AB29297), expandable thumbnail
  • Western blot - Mouse lung normal tissue lysate - total protein (AB29297), expandable thumbnail
  • Western blot - Mouse lung normal tissue lysate - total protein (AB29297), expandable thumbnail

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Liquid

Target data

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Mouse lung normal tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Liquid
Concentration
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Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting

Notes


Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

Ethics:
Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us.

Donor information can be supplied on request.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-RAGE antibody ab30381 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-RAGE antibody (Anti-RAGE antibody ab30381) at 1 µg/mL

    All lanes: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa

    Observed band size: 42 kDa, 50 kDa

    Exposure time: 1min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    The predicted molecular weight of GOLPH3/MIDAS is 34-kDa. We are unsure why we are seeing an additional band in HeLa and A549 whole cell lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    All lanes: Western blot - Anti-GOLPH3/MIDAS antibody (Anti-GOLPH3/MIDAS antibody ab98023) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: Lung (Human) Tissue Lysate at 10 µg

    Lane 3: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Lane 4: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 5: Lung (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Observed band size: 32 kDa, 34 kDa

    Exposure time: 30s

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Elf4/MEF antibody (Anti-Elf4/MEF antibody ab96075) at 1 µg/mL

    Lane 1: Testis (Mouse) Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 3: Spleen (Mouse) Tissue Lysate at 10 µg

    Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 5: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 71 kDa

    Observed band size: 55 kDa, 74 kDa

    Exposure time: 4min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Prostaglandin D2 Receptor antibody (Anti-Prostaglandin D2 Receptor antibody ab99446) at 1 µg/mL

    Lane 1: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 2: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 3: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 5: Western blot - EL4 whole cell lysate (EL4 whole cell lysate ab7183) at 10 µg

    Lane 6: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa

    Observed band size: 40 kDa, 87 kDa

    Exposure time: 4min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    ADAM33 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    The band observed at 68 kDa could potentially be a cleaved form of ADAM33 due to the presence of both a signal and pro peptide.
    The expression profile observed is consistent with what has been described in the literature (PMID:
    PMC2660367).

    All lanes: Western blot - Anti-ADAM33 antibody (Anti-ADAM33 antibody ab113740) at 1 µg/mL

    Lane 1: Mouse Placenta Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 3: Spleen (Mouse) Tissue Lysate at 10 µg

    Lane 4: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa

    Observed band size: 108 kDa, 28 kDa, 42 kDa, 68 kDa, 80 kDa

    Exposure time: 8min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    RPC6 contains a potential glycosylation site (SwissProt) which may explain the higher migration pattern observed.
    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    All lanes: Anti-TRPC6 antibody (ab101622) at 1 µg/mL

    Lane 1: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 2: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 3: Brain (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Mannose Receptor antibody (Anti-Mannose Receptor antibody ab64693) at 1 µg/mL

    Lane 1: Rat liver tissue lysate at 10 µg

    Lane 2: Human lung tissue lysate at 10 µg

    Lane 3: Rat lung tissue lysate at 10 µg

    Lane 4: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Secondary

    All lanes: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa

    Observed band size: 190 kDa

    Exposure time: 4min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-RhoA antibody (Anti-RhoA antibody ab86297) at 1 µg/mL

    Lane 1: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 3: Kidney (Rat) Tissue Lysate at 10 µg

    Lane 4: Lung (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 20 kDa

    Exposure time: 2min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody ab85445) at 1 µg/mL

    Lane 1: Kidney (Rat) Tissue Lysate at 10 µg

    Lane 2: Lung (Rat) Tissue Lysate at 10 µg

    Lane 3: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 4: Heart (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 77 kDa

    Observed band size: 48 kDa, 58 kDa, 77 kDa

    Exposure time: 3min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    Wnt9a contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-Wnt9a antibody ab125957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    All lanes: Western blot - Anti-Wnt9a antibody (Anti-Wnt9a antibody ab125957) at 1 µg/mL

    Lane 1: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 2: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 3: Western blot - T-47D whole cell lysate (T-47D whole cell lysate ab14899) at 10 µg

    Lane 4: PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa

    Observed band size: 110 kDa, 43 kDa, 70 kDa

    Exposure time: 1min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Choline kinase alpha antibody (Anti-Choline kinase alpha antibody ab88053) at 1 µg/mL

    Lane 1: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 3: Brain (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa

    Observed band size: 52 kDa, 72 kDa, 78 kDa

    Exposure time: 1min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Strumpellin antibody (Anti-Strumpellin antibody ab101222) at 1 µg/mL

    Lane 1: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 2: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 3: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 134 kDa

    Observed band size: 134 kDa, 45 kDa, 50 kDa, 55 kDa, 80 kDa

    Exposure time: 3min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (Anti-Heme Oxygenase 1 antibody ab85309) at 1 µg/mL

    Lane 1: Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Lane 2: Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281) at 10 µg

    Lane 3: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 4: Spleen (Mouse) Tissue Lysate at 10 µg

    Lane 5: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 30 kDa, 44 kDa

    Exposure time: 20min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody (Anti-Iroquois homeobox protein 3/IRX3 antibody ab25703) at 2 µg/mL

    Lane 1: Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 20 µg

    Lane 4: Spinal Cord (Mouse) Tissue Lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 52 kDa

    Observed band size: 52 kDa, 55 kDa

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    VCAM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    All lanes: Western blot - Anti-VCAM1 antibody (Anti-VCAM1 antibody ab115135) at 1 µg/mL

    Lane 1: Spleen (Mouse) Tissue Lysate at 10 µg

    Lane 2: Spleen (Rat) Tissue Lysate at 10 µg

    Lane 3: Thymus (Mouse) Tissue Lysate at 10 µg

    Lane 4: Thymus (Rat) Tissue Lysate at 10 µg

    Lane 5: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa

    Observed band size: 100 kDa, 62 kDa

    Exposure time: 4min

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    All lanes: Western blot - Anti-VCP antibody (Anti-VCP antibody ab36047) at 1 µg/mL

    Lane 1: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 2: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Lane 3: Liver (Mouse) Tissue Lysate at 10 µg

    Lane 4: Kidney (Rat) Tissue Lysate at 10 µg

    Lane 5: Lung (Rat) Tissue Lysate at 10 µg

    Lane 6: Liver (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 89 kDa

    Observed band size: 100 kDa, 52 kDa, 70 kDa

    Exposure time: 10s

  • Western blot - Mouse lung normal tissue lysate - total protein (ab29297), expandable thumbnail

    Western blot - Mouse lung normal tissue lysate - total protein (ab29297)

    The expression profile observed is consistent with what has been described in the literature PMID:15031288.
    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-RAIN antibody ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    All lanes: Western blot - Anti-RAIN antibody (Anti-RAIN antibody ab129820) at 1/250 dilution

    All lanes: Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 103 kDa

    Observed band size: 115 kDa, 23 kDa, 39 kDa, 68 kDa

    Exposure time: 8min

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For this product, it's our understanding that no specific protocols are required. You can:

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