Mouse lung normal tissue lysate - total protein

17 Images

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

The predicted molecular weight of GOLPH3/MIDAS is 34-kDa. We are unsure why we are seeing an additional band in HeLa and A549 whole cell lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-GOLPH3/MIDAS antibody (<a href='/en-us/products/primary-antibodies/golph3-midas-antibody-ab98023'>ab98023</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Lung (Human) Tissue Lysate at 10 µg

Lane 3:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 5:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 34 kDa

Observed band size: 32 kDa,34 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

VCAM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-VCAM1 antibody (<a href='/en-us/products/primary-antibodies/vcam1-antibody-ab115135'>ab115135</a>) at 1 µg/mL

Lane 1:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 2:

Spleen (Rat) Tissue Lysate at 10 µg

Lane 3:

Thymus (Mouse) Tissue Lysate at 10 µg

Lane 4:

Thymus (Rat) Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa,62 kDa

true

Exposure time: 4min

• WB

Ap

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30381 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-RAGE antibody (<a href='/en-us/products/primary-antibodies/rage-antibody-ab30381'>ab30381</a>) at 1 µg/mL

All lanes:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa,50 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

ADAM33 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of ADAM33 due to the presence of both a signal and pro peptide. The expression profile observed is consistent with what has been described in the literature (PMID : PMC2660367).

All lanes:

Western blot - Anti-ADAM33 antibody (<a href='/en-us/products/primary-antibodies/adam33-antibody-ab113740'>ab113740</a>) at 1 µg/mL

Lane 1:

Mouse Placenta Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 3:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 4:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 5:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 88 kDa

Observed band size: 108 kDa,28 kDa,42 kDa,68 kDa,80 kDa

true

Exposure time: 8min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Choline kinase alpha antibody (<a href='/en-us/products/primary-antibodies/choline-kinase-alpha-antibody-ab88053'>ab88053</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa,72 kDa,78 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

The expression profile observed is consistent with what has been described in the literature PMID : 15031288. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-RAIN antibody (<a href='/en-us/products/primary-antibodies/rain-antibody-ab129820'>ab129820</a>) at 1/250 dilution

All lanes:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 103 kDa

Observed band size: 115 kDa,23 kDa,39 kDa,68 kDa

true

Exposure time: 8min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-RhoA antibody (<a href='/en-us/products/primary-antibodies/rhoa-antibody-ab86297'>ab86297</a>) at 1 µg/mL

Lane 1:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 3:

Kidney (Rat) Tissue Lysate at 10 µg

Lane 4:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 22 kDa

Observed band size: 20 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-VCP antibody (<a href='/en-us/products/primary-antibodies/vcp-antibody-ab36047'>ab36047</a>) at 1 µg/mL

Lane 1:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 3:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 4:

Kidney (Rat) Tissue Lysate at 10 µg

Lane 5:

Lung (Rat) Tissue Lysate at 10 µg

Lane 6:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 89 kDa

Observed band size: 100 kDa,52 kDa,70 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Prostaglandin D2 Receptor antibody (<a href='/en-us/products/primary-antibodies/prostaglandin-d2-receptor-antibody-ab99446'>ab99446</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 5:

Western blot - EL4 whole cell lysate (<a href='/en-us/products/cell-lysates/el4-whole-cell-lysate-ab7183'>ab7183</a>) at 10 µg

Lane 6:

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa,87 kDa

true

Exposure time: 4min

• WB

Lab

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Mannose Receptor antibody (<a href='/en-us/products/primary-antibodies/mannose-receptor-antibody-ab64693'>ab64693</a>) at 1 µg/mL

Lane 1:

Rat liver tissue lysate at 10 µg

Lane 2:

Human lung tissue lysate at 10 µg

Lane 3:

Rat lung tissue lysate at 10 µg

Lane 4:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Secondary

All lanes:

Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 166 kDa

Observed band size: 190 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (<a href='/en-us/products/primary-antibodies/guanylyl-cyclase-alpha-1-gucy1a3-antibody-ab85445'>ab85445</a>) at 1 µg/mL

Lane 1:

Kidney (Rat) Tissue Lysate at 10 µg

Lane 2:

Lung (Rat) Tissue Lysate at 10 µg

Lane 3:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 4:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 77 kDa

Observed band size: 48 kDa,58 kDa,77 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Elf4/MEF antibody (<a href='/en-us/products/primary-antibodies/elf4-mef-antibody-ab96075'>ab96075</a>) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 3:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 5:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 71 kDa

Observed band size: 55 kDa,74 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Strumpellin antibody (<a href='/en-us/products/primary-antibodies/strumpellin-antibody-ab101222'>ab101222</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 3:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 134 kDa

Observed band size: 134 kDa,45 kDa,50 kDa,55 kDa,80 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (<a href='/en-us/products/primary-antibodies/heme-oxygenase-1-antibody-ab85309'>ab85309</a>) at 1 µg/mL

Lane 1:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Lane 2:

Human lung normal tissue lysate - total protein (40 - 65 years) (<a href='/en-us/products/unavailable/human-lung-normal-tissue-lysate-total-protein-40-65-years-ab30281'>ab30281</a>) at 10 µg

Lane 3:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 4:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 30 kDa,44 kDa

true

Exposure time: 20min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

RPC6 contains a potential glycosylation site (SwissProt) which may explain the higher migration pattern observed. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Anti-TRPC6 antibody (<a href='/en-us/products/unavailable/trpc6-antibody-ab101622'>ab101622</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 2:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 20min

• WB

Unknown

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

Wnt9a contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Wnt9a antibody (<a href='/en-us/products/primary-antibodies/wnt9a-antibody-ab125957'>ab125957</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Western blot - T-47D whole cell lysate (<a href='/en-us/products/cell-lysates/t-47d-whole-cell-lysate-ab14899'>ab14899</a>) at 10 µg

Lane 4:

PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 110 kDa,43 kDa,70 kDa

true

Exposure time: 1min

• WB

Project

Western blot - Mouse lung normal tissue lysate - total protein (AB29297)

All lanes:

Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody (<a href='/en-us/products/primary-antibodies/iroquois-homeobox-protein-3-irx3-antibody-ab25703'>ab25703</a>) at 2 µg/mL

Lane 1:

Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 4:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa,55 kDa

false