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Mouse pancreas tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Images

Western blot - Mouse pancreas tissue lysate - total protein (AB29363), expandable thumbnail
  • Western blot - Mouse pancreas tissue lysate - total protein (AB29363), expandable thumbnail
  • Western blot - Mouse pancreas tissue lysate - total protein (AB29363), expandable thumbnail
  • Western blot - Mouse pancreas tissue lysate - total protein (AB29363), expandable thumbnail
  • Western blot - Mouse pancreas tissue lysate - total protein (AB29363), expandable thumbnail

Key facts

Form
Liquid

Target data

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Mouse pancreas tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Liquid
Concentration
Loading...

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting

Notes


Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-Prohibitin antibody - Mitochondrial Marker (Anti-Prohibitin antibody - Mitochondrial Marker ab28172) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 5: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 6: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 8: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 10: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 11: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 12: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 13: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Lane 14: Liver (Rat) Tissue Lysate at 10 µg

    Lane 15: Heart (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 30 kDa

    Observed band size: 30 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-PARK7/DJ1 antibody (Anti-PARK7/DJ1 antibody ab18257) at 1 µg/mL

    Lane 1: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 2: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 3: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 4: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 5: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 6: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 7: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 8: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 9: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 10: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 11: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Lane 12: Liver (Rat) Tissue Lysate at 10 µg

    Lane 13: Heart (Rat) Tissue Lysate at 10 µg

    Lane 14: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa

    Observed band size: 15 kDa, 24 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-Calnexin antibody - ER Marker (Anti-Calnexin antibody - ER Marker ab22595) at 1/250 dilution

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg

    Lane 3: Brain (Mouse) Tissue Lysate (ab27253) at 10 µg

    Lane 4: Liver (Mouse) Tissue Lysate (ab7935) at 10 µg

    Lane 5: Heart (Mouse) Tissue Lysate (ab27255) at 10 µg

    Lane 6: Kidney (Mouse) Tissue Lysate (ab27254) at 10 µg

    Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 8: Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 10 µg

    Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 10: Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

    Lane 11: Ovary (Mouse) Tissue Lysate (ab35808) at 10 µg

    Lane 12: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg

    Lane 13: Brain (Rat) Tissue Lysate (ab7942) at 10 µg

    Lane 14: Liver (Rat) Tissue Lysate (ab27256) at 10 µg

    Lane 15: Heart (Rat) Tissue Lysate (ab7940) at 10 µg

    Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 68 kDa

    Observed band size: 80 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-PABP antibody (Anti-PABP antibody ab21060) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 6: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 7: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 8: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 9: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 71 kDa

    Observed band size: 110 kDa, 75 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-eIF4A1 antibody (Anti-eIF4A1 antibody ab31217) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    COQ10B has a predicted molecular weight of 27 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    All lanes: Anti-COQ10B antibody (ab41997) at 1 µg/mL

    Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: Liver (Rat) Tissue Lysate at 10 µg

    Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-EEF2/Elongation factor 2 antibody (Anti-EEF2/Elongation factor 2 antibody ab33523) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 4: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 6: Liver (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 95 kDa

    Observed band size: 95 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (Anti-eEF1A1+eEF1A2+eEF1AL3 antibody ab37969) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 5: Heart (Mouse) Tissue Lysate at 10 µg

    Lane 6: Kidney (Mouse) Tissue Lysate at 10 µg

    Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 8: Testis (Mouse) Tissue Lysate at 10 µg

    Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg

    Lane 10: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 11: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 12: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 13: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Lane 14: Liver (Rat) Tissue Lysate at 10 µg

    Lane 15: Heart (Rat) Tissue Lysate at 10 µg

    Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa

    Observed band size: 45 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-TCTP antibody (Anti-TCTP antibody ab37506) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 4: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 5: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 6: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 7: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 8: Liver (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa

    Observed band size: 22 kDa, 52 kDa

  • Western blot - Mouse pancreas tissue lysate - total protein (ab29363), expandable thumbnail

    Western blot - Mouse pancreas tissue lysate - total protein (ab29363)

    All lanes: Western blot - Anti-NUMBL antibody (Anti-NUMBL antibody ab37500) at 1 µg/mL

    Lane 1: Brain (Mouse) Whole Cell Lysate - normal tissue at 10 µg

    Lane 2: Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

    Lane 3: Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

    Lane 4: Lung (Mouse) Whole Cell Lysate - normal tissue at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 65 kDa

    Observed band size: 65 kDa, 70 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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