AB29363

Mouse pancreas tissue lysate - total protein

Name: Mouse pancreas tissue lysate - total protein (ab29363) | Abcam
Brand: Abcam Limited
SKU: AB29363

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Mouse pancreas tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

10 Images

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-eIF4A1 antibody (<a href='/en-us/products/primary-antibodies/eif4a1-antibody-ab31217'>ab31217</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 4:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 5:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

Observed band size: 47 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-Prohibitin antibody - Mitochondrial Marker (<a href='/en-us/products/primary-antibodies/prohibitin-antibody-mitochondrial-marker-ab28172'>ab28172</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 12:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-TCTP antibody (<a href='/en-us/products/primary-antibodies/tctp-antibody-ab37506'>ab37506</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 6:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 7:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa,52 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

COQ10B has a predicted molecular weight of 27 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Anti-COQ10B antibody (<a href='/en-us/products/unavailable/coq10b-antibody-ab41997'>ab41997</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Liver (Rat) Tissue Lysate at 10 µg

Lane 4:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

false

Unknown

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 antibody (<a href='/en-us/products/primary-antibodies/eef2-elongation-factor-2-antibody-ab33523'>ab33523</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 4:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Liver (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (<a href='/en-us/products/primary-antibodies/eef1a1eef1a2eef1al3-antibody-ab37969'>ab37969</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 12:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate at 10 µg

Lane 16:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 45 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-PARK7/DJ1 antibody (<a href='/en-us/products/primary-antibodies/park7-dj1-antibody-ab18257'>ab18257</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 3:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 4:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 6:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 7:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 8:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 9:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 10:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 11:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 12:

Liver (Rat) Tissue Lysate at 10 µg

Lane 13:

Heart (Rat) Tissue Lysate at 10 µg

Lane 14:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 15 kDa,24 kDa

false

Unknown

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-PABP antibody (<a href='/en-us/products/primary-antibodies/pabp-antibody-ab21060'>ab21060</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 5:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 7:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 8:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 9:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 71 kDa

Observed band size: 110 kDa,75 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-Calnexin antibody - ER Marker (<a href='/en-us/products/primary-antibodies/calnexin-antibody-er-marker-ab22595'>ab22595</a>) at 1/250 dilution

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate (ab27253) at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate (<a href='/en-us/products/unavailable/liver-mouse-tissue-lysate-ab7935'>ab7935</a>) at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate (ab27255) at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate (ab27254) at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate - normal tissue (<a href='/en-us/products/unavailable/testis-mouse-tissue-lysate-normal-tissue-ab4027'>ab4027</a>) at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate (ab35808) at 10 µg

Lane 12:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate (<a href='/en-us/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a>) at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate (ab27256) at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate (<a href='/en-us/products/unavailable/heart-rat-tissue-lysate-ab7940'>ab7940</a>) at 10 µg

Lane 16:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 80 kDa

false

Project

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

All lanes:

Western blot - Anti-NUMBL antibody (<a href='/en-us/products/primary-antibodies/numbl-antibody-ab37500'>ab37500</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 2:

Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 3:

Western blot - Mouse pancreas tissue lysate - total protein (ab29363) at 10 µg

Lane 4:

Lung (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa,70 kDa

false

Key facts

Form

Liquid

form

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

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Product details

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Allow to warm to room temp and agitate gently before aliquoting

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left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

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left-column

style

left-column

style

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse pancreas tissue lysate - total protein

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Western blot - Mouse pancreas tissue lysate - total protein (AB29363)

Key facts

Form

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Complementary Products

Secondary Antibodies

Cell Lines & Lysates

Cell Lines & Lysates

Primary Antibodies

Product promise