Mouse placenta normal tissue lysate - total protein

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• WB

Project

Western blot - Mouse placenta normal tissue lysate - total protein (AB29139)

ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.

All lanes:

Western blot - Anti-ADAMTS5 antibody (<a href='/en-us/products/primary-antibodies/adamts5-antibody-ab41037'>ab41037</a>) at 1/250 dilution

Lane 1:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Lane 2:

Western blot - Mouse placenta normal tissue lysate - total protein (ab29139) at 10 µg

Lane 3:

Uterus (Human) Whole Cell Lysate - normal tissue at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 27 kDa,73 kDa

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