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Mouse placenta normal tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Western blot - Mouse placenta normal tissue lysate - total protein (AB29139), expandable thumbnail

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Key facts

Form
Liquid

Target data

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Mouse placenta normal tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Liquid
Concentration
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Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting

Notes


Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

1 product image

  • Western blot - Mouse placenta normal tissue lysate - total protein (ab29139), expandable thumbnail

    Western blot - Mouse placenta normal tissue lysate - total protein (ab29139)

    Anti-ADAMTS5 antibody ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.

    All lanes: Western blot - Anti-ADAMTS5 antibody (Anti-ADAMTS5 antibody ab41037) at 1/250 dilution

    Lane 1: Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Lane 2: Western blot - Mouse placenta normal tissue lysate - total protein (ab29139) at 10 µg

    Lane 3: Uterus (Human) Whole Cell Lysate - normal tissue at 10 µg

    Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 102 kDa

    Observed band size: 27 kDa, 73 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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