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AB29711

Mouse skeletal muscle tissue lysate - total protein

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(2 Publications)

Mouse skeletal muscle tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
12 Images
Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-caveolae-marker-ab30750'>ab30750</a>) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 20 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 20 µg

Lane 3:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 20 µg

Lane 4:

Skeletal Muscle (Rat) Tissue Lysate - normal tissue (<a href='/en-us/products/unavailable/skeletal-muscle-rat-tissue-lysate-normal-tissue-ab29376'>ab29376</a>) at 20 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa,40 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-PDE5A/PDE5 antibody (<a href='/en-us/products/primary-antibodies/pde5a-pde5-antibody-ab64179'>ab64179</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 2:

Human ovary tissue lysate - total protein (<a href='/en-us/products/unavailable/human-ovary-tissue-lysate-total-protein-ab30222'>ab30222</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 100 kDa

Observed band size: 100 kDa,42 kDa,70 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-BAF53A antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/baf53a-antibody-chip-grade-ab3882'>ab3882</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 4:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 120 kDa,18 kDa,47 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Unknown

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727).

All lanes:

Western blot - Anti-FOXO3A antibody (<a href='/en-us/products/primary-antibodies/foxo3a-antibody-ab23683'>ab23683</a>) at 1 µg/mL

Lane 1:

Heart (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 2:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 3:

Heart (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 71 kDa

Observed band size: 16 kDa,27 kDa,90 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Ap

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody (<a href='/en-us/products/primary-antibodies/torsin-a-dyt1-antibody-ab34540'>ab34540</a>) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Mouse Brain at 10 µg

Lane 3:

Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 4:

Mouse Heart Mouse Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 6:

Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse brain tissue lysate - total protein (0 days) (<a href='/en-us/products/tissue-lysates/mouse-brain-tissue-lysate-total-protein-0-days-ab7188'>ab7188</a>) at 10 µg

Lane 8:

Mouse Embryonic Fibroblast 1 at 10 µg

Lane 9:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 38 kDa

Observed band size: 105 kDa,38 kDa,65 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-Prohibitin antibody - Mitochondrial Marker (<a href='/en-us/products/primary-antibodies/prohibitin-antibody-mitochondrial-marker-ab28172'>ab28172</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-pancreas-tissue-lysate-total-protein-ab29363'>ab29363</a>) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 12:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-FABP4 antibody (<a href='/en-us/products/primary-antibodies/fabp4-antibody-ab66682'>ab66682</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 2:

Western blot - Mouse thymus tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-thymus-tissue-lysate-total-protein-ab29285'>ab29285</a>) at 10 µg

Lane 3:

Rat Ovary Tissue Lysate at 10 µg

Lane 4:

Rat Colon Tissue Lysate at 10 µg

Lane 5:

Rat Thymus Tissue Lysate at 10 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa,18 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (<a href='/en-us/products/primary-antibodies/eef1a1eef1a2eef1al3-antibody-ab37969'>ab37969</a>) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-pancreas-tissue-lysate-total-protein-ab29363'>ab29363</a>) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 12:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate at 10 µg

Lane 16:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 45 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-eIF4A2 antibody (<a href='/en-us/products/primary-antibodies/eif4a2-antibody-ab31218'>ab31218</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 3:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 4:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 5:

Ovary (Mouse) Tissue Lysate at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Brain (Rat) Tissue Lysate at 10 µg

Lane 8:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

Observed band size: 47 kDa,51 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-PARK7/DJ1 antibody (<a href='/en-us/products/primary-antibodies/park7-dj1-antibody-ab18257'>ab18257</a>) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 3:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 4:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse pancreas tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-pancreas-tissue-lysate-total-protein-ab29363'>ab29363</a>) at 10 µg

Lane 6:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 7:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 8:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 9:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 10:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 11:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 12:

Liver (Rat) Tissue Lysate at 10 µg

Lane 13:

Heart (Rat) Tissue Lysate at 10 µg

Lane 14:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 15 kDa,24 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control (<a href='/en-us/products/primary-antibodies/vdac1-porin-vdac2-vdac3-antibody-mitochondrial-loading-control-ab15895'>ab15895</a>) at 1 µg/mL

Lane 1:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 2:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 3:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 4:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 5:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 7:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)
  • WB

Project

Western blot - Mouse skeletal muscle tissue lysate - total protein (AB29711)

All lanes:

Western blot - Anti-Calnexin antibody - ER Marker (<a href='/en-us/products/primary-antibodies/calnexin-antibody-er-marker-ab22595'>ab22595</a>) at 1/250 dilution

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate (ab27253) at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate (<a href='/en-us/products/unavailable/liver-mouse-tissue-lysate-ab7935'>ab7935</a>) at 10 µg

Lane 5:

Heart (Mouse) Tissue Lysate (ab27255) at 10 µg

Lane 6:

Kidney (Mouse) Tissue Lysate (ab27254) at 10 µg

Lane 7:

Western blot - Mouse pancreas tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-pancreas-tissue-lysate-total-protein-ab29363'>ab29363</a>) at 10 µg

Lane 8:

Testis (Mouse) Tissue Lysate - normal tissue (<a href='/en-us/products/unavailable/testis-mouse-tissue-lysate-normal-tissue-ab4027'>ab4027</a>) at 10 µg

Lane 9:

Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg

Lane 10:

Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

Lane 11:

Ovary (Mouse) Tissue Lysate (ab35808) at 10 µg

Lane 12:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg

Lane 13:

Brain (Rat) Tissue Lysate (<a href='/en-us/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a>) at 10 µg

Lane 14:

Liver (Rat) Tissue Lysate (ab27256) at 10 µg

Lane 15:

Heart (Rat) Tissue Lysate (<a href='/en-us/products/unavailable/heart-rat-tissue-lysate-ab7940'>ab7940</a>) at 10 µg

Lane 16:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 80 kDa

false

Key facts

Form

Liquid

form

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting
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Product protocols

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental gerontology 142:111104 PubMed33017670

2020

Myogenic marker expression as a function of age and exercise-based therapy in the tongue.

Applications

Unspecified application

Species

Unspecified reactive species

Heidi Kletzien,Cynthia A Kelm-Nelson,Sabrina Wang,Masatoshi Suzuki,Nadine P Connor

Dysphagia :918-934 PubMed32130514

2020

Effects of Tongue Exercise Frequency on Tongue Muscle Biology and Swallowing Physiology in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany N Krekeler,Jacqueline M Weycker,Nadine P Connor
View all publications
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