Mouse skeletal muscle tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- Experimental gerontology 142:1111042020Myogenic marker expression as a function of age and exercise-based therapy in the tongue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeidi Kletzien,Cynthia A Kelm-Nelson,Sabrina Wang,Masatoshi Suzuki,Nadine P ConnorPubMed 33017670
- Dysphagia :918-9342020Effects of Tongue Exercise Frequency on Tongue Muscle Biology and Swallowing Physiology in a Rat Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany N Krekeler,Jacqueline M Weycker,Nadine P ConnorPubMed 32130514
Key facts
- Form
- Liquid
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Mouse skeletal muscle tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Form
- Liquid
- Concentration
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Allow to warm to room temp and agitate gently before aliquoting
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12 product images
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-Prohibitin antibody - Mitochondrial Marker (Anti-Prohibitin antibody - Mitochondrial Marker ab28172) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: Heart (Mouse) Tissue Lysate at 10 µg
Lane 6: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 8: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 10: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 11: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 12: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 13: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 14: Liver (Rat) Tissue Lysate at 10 µg
Lane 15: Heart (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control (Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control ab15895) at 1 µg/mL
Lane 1: Heart (Mouse) Tissue Lysate at 10 µg
Lane 2: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 4: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 7: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-PARK7/DJ1 antibody (Anti-PARK7/DJ1 antibody ab18257) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 3: Heart (Mouse) Tissue Lysate at 10 µg
Lane 4: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 5: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 6: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 7: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 8: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 9: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 10: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 11: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 12: Liver (Rat) Tissue Lysate at 10 µg
Lane 13: Heart (Rat) Tissue Lysate at 10 µg
Lane 14: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 15 kDa, 24 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-eIF4A2 antibody (Anti-eIF4A2 antibody ab31218) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Testis (Mouse) Tissue Lysate at 10 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 4: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 5: Ovary (Mouse) Tissue Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 7: Brain (Rat) Tissue Lysate at 10 µg
Lane 8: Heart (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa, 51 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
Anti-FOXO3A antibody ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727).
All lanes: Western blot - Anti-FOXO3A antibody (Anti-FOXO3A antibody ab23683) at 1 µg/mL
Lane 1: Heart (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 2: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 3: Heart (Rat) Tissue Lysate - normal tissue at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 71 kDa
Observed band size: 16 kDa, 27 kDa, 90 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (Anti-Caveolin-3 antibody - Caveolae Marker ab30750) at 1 µg/mL
Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg
Lane 2: Human heart tissue lysate - total protein (ab29431) at 20 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 20 µg
Lane 4: Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376) at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa, 40 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-PDE5A/PDE5 antibody (Anti-PDE5A/PDE5 antibody ab64179) at 1 µg/mL
Lane 1: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 2: Human ovary tissue lysate - total protein (ab30222) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa, 42 kDa, 70 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-Torsin A/DYT1 antibody (Anti-Torsin A/DYT1 antibody ab34540) at 1 µg/mL
Lane 1: Marker
Lane 2: Mouse Brain at 10 µg
Lane 3: Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
Lane 4: Mouse Heart Mouse Tissue Lysate at 10 µg
Lane 5: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 6: Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
Lane 7: Western blot - Mouse brain tissue lysate - total protein (0 days) (Mouse brain tissue lysate - total protein (0 days) ab7188) at 10 µg
Lane 8: Mouse Embryonic Fibroblast 1 at 10 µg
Lane 9: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 105 kDa, 38 kDa, 65 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-BAF53A antibody - ChIP Grade (Anti-BAF53A antibody - ChIP Grade ab3882) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 4: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 120 kDa, 18 kDa, 47 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (Anti-eEF1A1+eEF1A2+eEF1AL3 antibody ab37969) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: Heart (Mouse) Tissue Lysate at 10 µg
Lane 6: Kidney (Mouse) Tissue Lysate at 10 µg
Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 8: Testis (Mouse) Tissue Lysate at 10 µg
Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 10: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 11: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 12: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 13: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 14: Liver (Rat) Tissue Lysate at 10 µg
Lane 15: Heart (Rat) Tissue Lysate at 10 µg
Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-FABP4 antibody (Anti-FABP4 antibody ab66682) at 1 µg/mL
Lane 1: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 2: Western blot - Mouse thymus tissue lysate - total protein (Mouse thymus tissue lysate - total protein ab29285) at 10 µg
Lane 3: Rat Ovary Tissue Lysate at 10 µg
Lane 4: Rat Colon Tissue Lysate at 10 µg
Lane 5: Rat Thymus Tissue Lysate at 10 µg
Lane 6: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa, 18 kDa
Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711)
All lanes: Western blot - Anti-Calnexin antibody - ER Marker (Anti-Calnexin antibody - ER Marker ab22595) at 1/250 dilution
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770) at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate (ab27253) at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate (ab7935) at 10 µg
Lane 5: Heart (Mouse) Tissue Lysate (ab27255) at 10 µg
Lane 6: Kidney (Mouse) Tissue Lysate (ab27254) at 10 µg
Lane 7: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 8: Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 10 µg
Lane 9: Western blot - Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 10: Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg
Lane 11: Ovary (Mouse) Tissue Lysate (ab35808) at 10 µg
Lane 12: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) at 10 µg
Lane 13: Brain (Rat) Tissue Lysate (ab7942) at 10 µg
Lane 14: Liver (Rat) Tissue Lysate (ab27256) at 10 µg
Lane 15: Heart (Rat) Tissue Lysate (ab7940) at 10 µg
Lane 16: Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa
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