Mouse skin tissue lysate (14 days)
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- WB
Unknown
Western blot - Mouse skin tissue lysate (14 days) (AB7271)
All lanes:
Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (<a href='/en-us/products/primary-antibodies/hair-cortex-cytokeratin-k40-antibody-ae13-ab16113'>ab16113</a>) at 1 µg/mL
Lane 1:
Western blot - Mouse skin tissue lysate (14 days) (ab7271) at 10 µg
Lane 2:
Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 25 kDa,44 kDa,55 kDa
true
Exposure time: 20min
Complementary Products
Reactivity data
Product details
Source: Outbred NIH-Swiss Albino mice (14 days old)
The mouse skin tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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