Mouse skin tissue lysate - total protein
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(0 Publication)
- WB
Unknown
Western blot - Mouse skin tissue lysate - total protein (AB4025)
All lanes:
Western blot - Anti-p92 antibody (<a href='/en-us/products/primary-antibodies/p92-antibody-ab72210'>ab72210</a>) at 1 µg/mL
Lane 1:
Brain (Mouse) Tissue Lysate at 10 µg
Lane 2:
Western blot - Mouse skin tissue lysate - total protein (ab4025) at 10 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 92 kDa
Observed band size: 105 kDa,52 kDa,92 kDa
true
- WB
Project
Western blot - Mouse skin tissue lysate - total protein (AB4025)
All lanes:
Western blot - Anti-SLUG antibody [mAbcam51772] (<a href='/en-us/products/primary-antibodies/slug-antibody-mabcam51772-ab51772'>ab51772</a>) at 5 µg/mL
All lanes:
Western blot - Mouse skin tissue lysate - total protein (ab4025) at 10 µg
Secondary
All lanes:
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa,34 kDa,60 kDa
false
Exposure time: 10min
Reactivity data
Product details
Source: Outbred NIH-Swiss Albino mice.
Mouse skin tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecyl sulfate (SDS), 1 mM sodium ethylene diamine tetra acetate, 1 mM phenyl methyl sulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
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