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AB29285

Mouse thymus tissue lysate - total protein

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(1 Publication)

Mouse thymus tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
2 Images
Western blot - Mouse thymus tissue lysate - total protein (AB29285)
  • WB

Project

Western blot - Mouse thymus tissue lysate - total protein (AB29285)

All lanes:

Western blot - Anti-SOCS2 antibody (<a href='/en-us/products/primary-antibodies/socs2-antibody-ab66733'>ab66733</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - SW480 whole cell lysate (<a href='/en-us/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Lane 4:

Western blot - Raji whole cell lysate (<a href='/en-us/products/cell-lysates/raji-whole-cell-lysate-ab30124'>ab30124</a>) at 10 µg

Lane 5:

Western blot - Mouse thymus tissue lysate - total protein (ab29285) at 10 µg

Lane 6:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Western blot - Mouse thymus tissue lysate - total protein (AB29285)
  • WB

Project

Western blot - Mouse thymus tissue lysate - total protein (AB29285)

All lanes:

Western blot - Anti-FABP4 antibody (<a href='/en-us/products/primary-antibodies/fabp4-antibody-ab66682'>ab66682</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 2:

Western blot - Mouse thymus tissue lysate - total protein (ab29285) at 10 µg

Lane 3:

Rat Ovary Tissue Lysate at 10 µg

Lane 4:

Rat Colon Tissue Lysate at 10 µg

Lane 5:

Rat Thymus Tissue Lysate at 10 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa,18 kDa

false

Key facts

Form

Liquid

form

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

Product details

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:2002 PubMed33133060

2020

Increased Macrophages and C1qA, C3, C4 Transcripts in the Midbrain of People With Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Tertia D Purves-Tyson,Kate Robinson,Amelia M Brown,Danny Boerrigter,Helen Q Cai,Christin Weissleder,Samantha J Owens,Debora A Rothmond,Cynthia Shannon Weickert
View all publications

Product promise

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