Mouse thymus tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- Frontiers in immunology 11:20022020Increased Macrophages and C1qA, C3, C4 Transcripts in the Midbrain of People With Schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTertia D Purves-Tyson et. al.PubMed 33133060
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Mouse thymus tissue lysate - total protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Form
- Liquid
- Concentration
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Allow to warm to room temp and agitate gently before aliquoting
Notes
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Mouse thymus tissue lysate - total protein (ab29285)
All lanes: Western blot - Anti-SOCS2 antibody (Anti-SOCS2 antibody ab66733) at 1 µg/mL
Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Western blot - SW480 whole cell lysate (SW480 whole cell lysate ab3957) at 10 µg
Lane 4: Western blot - Raji whole cell lysate (Raji whole cell lysate ab30124) at 10 µg
Lane 5: Western blot - Mouse thymus tissue lysate - total protein (ab29285) at 10 µg
Lane 6: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Western blot - Mouse thymus tissue lysate - total protein (ab29285)
All lanes: Western blot - Anti-FABP4 antibody (Anti-FABP4 antibody ab66682) at 1 µg/mL
Lane 1: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 2: Western blot - Mouse thymus tissue lysate - total protein (ab29285) at 10 µg
Lane 3: Rat Ovary Tissue Lysate at 10 µg
Lane 4: Rat Colon Tissue Lysate at 10 µg
Lane 5: Rat Thymus Tissue Lysate at 10 µg
Lane 6: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa, 18 kDa
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